主要的2型人类免疫缺陷病毒（HIV-2）包装信号存在于所有HIV-2 RNA种类中：共翻译RNA衣壳化以及Gag蛋白的限制赋予了特异性。
The major human immunodeficiency virus type 2 (HIV-2) packaging signal is present on all HIV-2 RNA species: cotranslational RNA encapsidation and limitation of Gag protein confer specificity.
作者信息
Griffin S D, Allen J F, Lever A M
机构信息
Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 2QQ, United Kingdom.
出版信息
J Virol. 2001 Dec;75(24):12058-69. doi: 10.1128/JVI.75.24.12058-12069.2001.
Abstract
Deletion of a region of the human immunodeficiency virus type 2 (HIV-2) 5' leader RNA reduces genomic RNA encapsidation to about 5% that of wild-type virus with no defect in viral protein production but severely limits virus spread in Jurkat T cells, indicating that this region contains a major cis-acting encapsidation signal, or psi (Psi). Being upstream of the major splice donor, it is present on all viral transcripts. We have shown that HIV-2 selects its genomic RNA for encapsidation cotranslationally, rendering wild-type HIV-2 unable to encapsidate vector RNAs in trans. Virus with Psi deleted, however, encapsidates an HIV-2 vector, demonstrating competition for Gag protein. HIV-2 overcomes the lack of packaging signal location specificity by two novel mechanisms, cotranslational packaging and competition for limiting Gag polyprotein.
摘要
删除人类免疫缺陷病毒2型（HIV-2）5'前导RNA的一个区域会使基因组RNA的包装率降至野生型病毒的约5%，病毒蛋白产生无缺陷，但严重限制了病毒在Jurkat T细胞中的传播，这表明该区域包含一个主要的顺式作用包装信号，即ψ（Psi）。由于它位于主要剪接供体的上游，所以存在于所有病毒转录本上。我们已经表明，HIV-2在共翻译时选择其基因组RNA进行包装，这使得野生型HIV-2无法反式包装载体RNA。然而，删除了Psi的病毒能够包装HIV-2载体，这表明存在对Gag蛋白的竞争。HIV-2通过两种新机制克服了包装信号位置特异性的缺乏，即共翻译包装和对有限Gag多聚蛋白的竞争。
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