Moné M J, Volker M, Nikaido O, Mullenders L H, van Zeeland A A, Verschure P J, Manders E M, van Driel R
Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands.
EMBO Rep. 2001 Nov;2(11):1013-7. doi: 10.1093/embo-reports/kve224.
UV-induced DNA damage causes cells to repress RNA synthesis and to initiate nucleotide excision repair (NER). NER and transcription are intimately linked processes. Evidence has been presented that, in addition to damaged genes, undamaged loci are transcriptionally inhibited. We investigated whether RNA synthesis from undamaged genes is affected by the presence of UV damage elsewhere in the same nucleus, using a novel technique to UV irradiate only part of a nucleus. We show that the basal transcription/repair factor TFIIH is recruited to the damaged nuclear area, partially depleting the undamaged nuclear area. Remarkably, this sequestration has no effect on RNA synthesis. This result was obtained for cells that are able to carry out NER and for cells deficient in NER. We conclude that cross talk between NER and transcription occurs only over short distances in nuclei of living cells.
紫外线诱导的DNA损伤会导致细胞抑制RNA合成并启动核苷酸切除修复（NER）。NER和转录是紧密相连的过程。已有证据表明，除了受损基因外，未受损的基因座也会受到转录抑制。我们使用一种仅对细胞核的一部分进行紫外线照射的新技术，研究了来自未受损基因的RNA合成是否会受到同一细胞核中其他位置紫外线损伤的影响。我们发现，基础转录/修复因子TFIIH会被招募到受损的核区域，从而使未受损的核区域部分耗尽。值得注意的是，这种隔离对RNA合成没有影响。无论是能够进行NER的细胞还是NER缺陷的细胞，都得到了这一结果。我们得出结论，在活细胞的细胞核中，NER和转录之间的相互作用仅在短距离内发生。
