荚膜红细菌中的一种信使核糖核酸降解复合体。
An mRNA degrading complex in Rhodobacter capsulatus.
作者信息
Jäger S, Fuhrmann O, Heck C, Hebermehl M, Schiltz E, Rauhut R, Klug G
机构信息
Institut für Mikro- und Molekularbiologie, Justus Liebig Universität Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
出版信息
Nucleic Acids Res. 2001 Nov 15;29(22):4581-8. doi: 10.1093/nar/29.22.4581.
Abstract
An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.
摘要
从荚膜红细菌中纯化出一种具有RNA降解功能的高分子量复合物。利用N端测序、甘油梯度离心、免疫亲和纯化以及功能测定来确定该复合物的物理和生化特性。该复合物包含核糖核酸酶E以及分别为74 kDa和65 kDa的两种DEAD盒RNA解旋酶。最令人惊讶的是，转录终止因子Rho是降解体的主要且紧密结合的组分。
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本文引用的文献
1
From RNA helicases to RNPases.从RNA解旋酶到核糖核酸酶。
Trends Biochem Sci. 2001 Jun;26(6):339-41. doi: 10.1016/s0968-0004(01)01870-9.
2
Musing on the structural organization of the exosome complex.关于外泌体复合体结构组织的思考。
Nat Struct Biol. 2000 Oct;7(10):843-6. doi: 10.1038/82817.
3
Emerging features of mRNA decay in bacteria.细菌中mRNA衰变的新特征。
RNA. 2000 Aug;6(8):1079-90. doi: 10.1017/s1355838200001023.
4
mRNA stability in eukaryotes.真核生物中的信使核糖核酸稳定性
Curr Opin Genet Dev. 2000 Apr;10(2):193-8. doi: 10.1016/s0959-437x(00)00063-0.
5
The CafA protein required for the 5'-maturation of 16 S rRNA is a 5'-end-dependent ribonuclease that has context-dependent broad sequence specificity.16 S rRNA 5'-成熟所需的CafA蛋白是一种5'-末端依赖性核糖核酸酶，具有上下文依赖性的广泛序列特异性。
J Biol Chem. 2000 Mar 24;275(12):8726-32. doi: 10.1074/jbc.275.12.8726.
6
mRNA degradation in bacteria.细菌中的信使核糖核酸降解
FEMS Microbiol Rev. 1999 Jun;23(3):353-70. doi: 10.1111/j.1574-6976.1999.tb00404.x.
7
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EMBO J. 1999 May 17;18(10):2878-85. doi: 10.1093/emboj/18.10.2878.
8
Degrading chloroplast mRNA: the role of polyadenylation.降解叶绿体mRNA：多聚腺苷酸化的作用
Trends Biochem Sci. 1999 May;24(5):199-202. doi: 10.1016/s0968-0004(99)01388-2.
9
The structural basis for terminator recognition by the Rho transcription termination factor.
Mol Cell. 1999 Apr;3(4):487-93. doi: 10.1016/s1097-2765(00)80476-1.
10
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