单克隆抗胶质纤维酸性蛋白抗体：提取、特性及免疫酶测定

Monoclonal anti-GFAP antibodies: extraction, characteristics, and immunoenzyme assay.

作者信息

Chekhonin V P, Gurina O I, Dmitrieva T B, Shepeleva I I, Ryabukhin I A, Babich G N

机构信息

Laboratory of Immunochemistry, V. P. Serbskii State Research Center of Social and Forensis Psychiatry, Moscow.

出版信息

Bull Exp Biol Med. 2001 Aug;132(2):772-5. doi: 10.1023/a:1013090214269.

DOI:10.1023/a:1013090214269

PMID:11713563

Abstract

Methods of GFAP purification and obtaining of hybridoma cells producing monoclonal anti-GFAP antibodies and properties of GFAP preparation were described. The immunobloting data on specificity of obtained monoclonal antibodies are presented. A new method of GFAP immunoenzyme assay based on GFAP preparation and anti-GFAP antibodies was elaborated. Standardization of the immunoenzyme system was shown in tests for specificity, accuracy, and reproducibility.

摘要

描述了胶质纤维酸性蛋白（GFAP）的纯化方法、产生抗GFAP单克隆抗体的杂交瘤细胞的制备方法以及GFAP制剂的特性。给出了关于所获单克隆抗体特异性的免疫印迹数据。阐述了一种基于GFAP制剂和抗GFAP抗体的GFAP免疫酶测定新方法。在特异性、准确性和可重复性测试中展示了免疫酶系统的标准化。