Eliakim R, Karmeli F, Rachmilewitz D, Cohen P, Zimran A
Department of Medicine, Hadassah University Hospital, Jerusalem, Israel.
Dig Dis Sci. 2001 Nov;46(11):2515-20. doi: 10.1023/a:1012348525208.
Ozone is one of the most powerful oxidants available, with many applications in industry and medicine. Medically relevant features of ozone include bacterial and virucidal properties, disinfection, sterilization, circulatory stimulation, and disruption of malignant cells. Ozone therapy is administered in various ways, including intravenously, intramuscularly, and intrarectally. The latter modality is used for the treatment of colitis and hepatitis. Our aim was to examine the effect of ozone water enema on normal and inflamed rat colonic mucosa. Ozone water (20 microg/ml) was prepared via ozone generator and administered intrarectally (0.5 ml) daily. Rats were killed one, three, and seven days after rectal ozone water administration, and their colons resected, rinsed, and weighed (grams per 10 cm). Damage was assessed macro- and microscopically and tissue processed for myeloperoxidase and nitric oxide synthase activity. Rats receiving saline served as controls. In an additional experiment colitis was induced by intrarectal iodoacetamide. Ozone therapy caused no macroscopic damage. Ozone therapy induced microscopic colitis, which lasted for at least a week and was accompanied by increase in segmental weight, myeloperoxidase and nitric oxide activity, and prostaglandin E2 generation. Ozone therapy had no protective effect on inflamed mucosa. In conclusion, ozone water therapy had a deleterious effect on normal colonic mucosa, suggesting intrarectal administration be reevaluated. Ozone water enema may serve as a model of microscopic colitis.
臭氧是现有的最强氧化剂之一，在工业和医学领域有诸多应用。臭氧的医学相关特性包括杀菌和杀病毒特性、消毒、灭菌、循环刺激以及破坏恶性细胞。臭氧疗法有多种给药方式，包括静脉注射、肌肉注射和直肠给药。后一种方式用于治疗结肠炎和肝炎。我们的目的是研究臭氧水灌肠对正常和发炎大鼠结肠黏膜的影响。通过臭氧发生器制备臭氧水（20微克/毫升），每天直肠给药（0.5毫升）。在直肠给予臭氧水后的第1、3和7天处死大鼠，切除其结肠，冲洗并称重（每10厘米克数）。通过宏观和微观评估损伤情况，并对组织进行髓过氧化物酶和一氧化氮合酶活性检测。接受生理盐水的大鼠作为对照。在另一项实验中，通过直肠注射碘乙酰胺诱导结肠炎。臭氧疗法未造成宏观损伤。臭氧疗法诱发了微观结肠炎，持续至少一周，并伴有节段重量增加、髓过氧化物酶和一氧化氮活性增加以及前列腺素E2生成。臭氧疗法对发炎黏膜没有保护作用。总之，臭氧水疗法对正常结肠黏膜有有害影响，提示直肠给药方式需重新评估。臭氧水灌肠可作为微观结肠炎的一个模型。
