Schierhorn K, Zhang M, Kacy M, Kunkel G
Department of Clinical Immunology and Asthma OPD, Virchow-Klinikum, Humboldt University Berlin, Germany.
Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):312-5. doi: 10.1159/000237585.
The increase in airway responsiveness induced by ozone exposure is associated with airway inflammation as evidenced by an increase in inflammatory mediators such as cyclooxygenase and lipoxygenase product formation found in bronchoalveolar lavage fluid, nasal lavage fluid and epithelial cell cultures. To examine eicosanoid metabolism after exposure to ozone, human nasal mucosa derived from 21 patients undergoing surgical therapy for chronic nasal obstruction was cultured with a specially designed in vitro organ culture device and exposed to 0.1 ppm ozone for 24 h. Eicosanoid formation was analyzed by the release of cyclooxygenase and lipoxygenase products into the culture supernatant (measured by enzyme immunoassay). Experiments revealed ozone-induced increases in cyclooxygenase and lipoxygenase product formation with significant increases in prostaglandin F2alpha (PGF2alpha), thromboxane B2 (TXB2) and leukotriene B4 (LTB4) (p<0.05). Moreover, we found an increase in the concentration of LTC4/D4/E4 in the supernatant of ozone-exposed mucosal samples, which however does not reach statistical significance. There was a significant correlation between PGF2alpha and TXB2 (r = 0.71, p<0.001). These results extend previous results from in vivo chamber studies suggesting that the mode of action of ozone is an oxidative reaction resulting in an increased activity of arachidonic acid metabolism in the airways with a subsequent increase in the concentration of cyclooxygenase and lipoxygenase products.
臭氧暴露引起的气道反应性增加与气道炎症相关，支气管肺泡灌洗液、鼻腔灌洗液和上皮细胞培养物中发现的炎症介质如环氧化酶和脂氧化酶产物形成增加就证明了这一点。为了研究暴露于臭氧后的类花生酸代谢，将来自21例因慢性鼻阻塞接受手术治疗患者的人鼻黏膜，用专门设计的体外器官培养装置进行培养，并暴露于0.1 ppm臭氧中24小时。通过环氧化酶和脂氧化酶产物释放到培养上清液中（通过酶免疫测定法测量）来分析类花生酸的形成。实验显示，臭氧诱导环氧化酶和脂氧化酶产物形成增加，前列腺素F2α（PGF2α）、血栓素B2（TXB2）和白三烯B4（LTB4）显著增加（p<0.05）。此外，我们发现臭氧暴露的黏膜样品上清液中LTC4/D4/E4浓度增加，然而未达到统计学意义。PGF2α和TXB2之间存在显著相关性（r = 0.71，p<0.001）。这些结果扩展了先前体内实验舱研究的结果，表明臭氧的作用方式是氧化反应，导致气道中花生四烯酸代谢活性增加，随后环氧化酶和脂氧化酶产物浓度增加。
