[IgA肾病与乙肝病毒感染之间的关系]

[The relationship between IgA nephropathy and HBV infection].

作者信息

Ma X, Zhang Y, Du W

机构信息

Department of Pathology, Shanghai Medical University, Shanghai 200032, China.

出版信息

Zhonghua Yi Xue Za Zhi. 1999 Jun;79(6):417-21.

PMID:11715434

Abstract

OBJECTIVE

To clarify the correlation between hepatitis B virus (HBV) infection and the pathogenesis of IgA nephropathy.

METHODS

Ninety-one cases of primary IgA nephropathy were investigated by using in situ hybridization(HBV DNA), immunohistochemistry (HBAg) and HBV DNA-HBAg double staining techniques. In some of the cases, Southern blot analysis and serologic HBV infectious markers were detected also.

RESULTS

The positive rate of HBAg in renal tissue detected by immunohistochemistry was 69% (63/91) and that of the HBV DNA detected by in situ hybridization was 43% (39/91). Eighteen of 27 cases showed HBV DNA positive in Southern blot analysis, and all of the positive cases were identified to be the integrated form. Among them, 17 cases were HBAg detectable in renal tissue by immunohistochemistry and 16 cases were HBV DNA detectable by in situ hybridization simultaneously. The positive rate of the expression of HBcAg [77% (30/39)] and HBsAg [72% (28/39)] of the tubular epithelial cells with HBV DNA signals in nuclears by in situ hybridization was significantly higher than that of the cells without HBV DNA signals(10%, 21%). The double staining showed that most of the HBV DNA positive tubular epithelial cells coexpressed HBsAg and(or) HBcAg.

CONCLUSION

The renal tissue of some of IgA nephropathy infected with HBV directly and express HBAg in situ.

摘要

目的

阐明乙型肝炎病毒（HBV）感染与IgA肾病发病机制之间的相关性。

方法

采用原位杂交（检测HBV DNA）、免疫组织化学（检测HBsAg）及HBV DNA-HBsAg双重染色技术，对91例原发性IgA肾病患者进行研究。部分病例还检测了Southern印迹分析及血清学HBV感染标志物。

结果

免疫组织化学检测肾组织中HBsAg阳性率为69%（63/91），原位杂交检测HBV DNA阳性率为43%（39/91）。Southern印迹分析27例中有18例HBV DNA阳性，且所有阳性病例均为整合型。其中，17例免疫组织化学检测肾组织中可检测到HBsAg，16例原位杂交可检测到HBV DNA。原位杂交显示，细胞核中有HBV DNA信号的肾小管上皮细胞中HBcAg[77%（30/39）]和HBsAg[72%（28/39）]的表达阳性率显著高于无HBV DNA信号的细胞（分别为10%、21%）。双重染色显示，大多数HBV DNA阳性的肾小管上皮细胞共表达HBsAg和（或）HBcAg。