Chen N, Wang Z, Ren H
Department of Nephrology, Ruijin Hospital, Shanghai 2nd Medical University, Shanghai 200025, China.
Zhonghua Yi Xue Za Zhi. 2001 Nov 10;81(21):1309-12.
To study the presence and pathogenesis of hepatitis B virus (HBV) DNA and RNA in renal tissues.
The presence and distribution of HBV DNA and RNA were investigated in renal biopsy tissues of 27 patients with HBV associated glomerulonephritis by in situ hybridization assay to explore the pathogenesis of HBV in renal tissue.
HBV DNA and RNA, the same as HBV antigens, were present in renal tissues and located in the nuclei and cytoplasm of renal tubular epithelial cells, glomerular mesangium and capillary loops. HBV DNA and RNA were detected in tubular epithelia with the positive rates of 81.5%, 63.0% respectively, which were significantly higher than those in glomeruli (48.1%, 25.9% respectively) (P < 0.05).
That HBV exists and replicates too in renal tissues, predominantly in tubular epithelia. The HBV antigens expressed in situ, as well as the antigens from circulation, and lead to renal lesions through immune complex formation or cell immune involvement. Besides glomerular lesions, tubulointerstitial lesions might also determine the progression and prognosis of HBV associated glomerulonephritis.
研究肾组织中乙型肝炎病毒（HBV）DNA和RNA的存在情况及发病机制。
采用原位杂交法检测27例HBV相关性肾小球肾炎患者肾活检组织中HBV DNA和RNA的存在及分布情况，以探讨HBV在肾组织中的发病机制。
肾组织中存在HBV DNA和RNA，与HBV抗原一样，位于肾小管上皮细胞、肾小球系膜及毛细血管袢的细胞核和细胞质中。在肾小管上皮细胞中检测到HBV DNA和RNA，阳性率分别为81.5%、63.0%，显著高于肾小球中的阳性率（分别为48.1%、25.9%）（P<0.05）。
HBV在肾组织中存在且复制，主要存在于肾小管上皮细胞中。原位表达的HBV抗原以及循环中的抗原，通过免疫复合物形成或细胞免疫参与导致肾损害。除肾小球病变外，肾小管间质病变也可能决定HBV相关性肾小球肾炎的进展和预后。
