[乙型肝炎病毒感染在IgA肾病发病机制中的作用]

[Role of hepatitis B virus infection in pathogenesis of IgA nephropathy].

作者信息

Wang Nian-song, Wu Zhao-long, Zhang Yue-e, Guo Mu-yi, Liao Lü-tan

机构信息

Department of Nephrology, The Sixth People's Hospital of Shanghai, Shanghai 200233, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Jan;37(1):37-40.

PMID:12760794

Abstract

OBJECTIVE

To clarify the relationship between hepatitis B virus (HBV) infection and IgA nephropathy (IgAN).

METHODS

HBV antigen (HBAg) in renal tissues of the patients with IgAN was detected by immunohistochemical technique, the carrier status and localization of HBV DNA in renal tissues were determined by Southern blot analysis and in situ hybridization.

RESULTS

Serum HBsAg was detected in 18 of the 100 patients with IgAN (18%), HBAg was detected in 31 of 100 patients (31%) in their renal tissue and in 20 of 31 patients (65%) in their glomeruli, and both HBsAg and HBcAg were detected in 10 of 31 patients (32%), respectively. HBcAg was also found in tubular epithelia (45%, 14/31) and renal interstitium (6%, 2/31), respectively. Five of six cases were proved to be positive of integrated-form HBV DNA in their renal tissue by Southern blot analysis. In situ hybridization demonstrated that HBV DNA was 8/8 and 6/8 positive in their renal tubules and glomeruli of all eight specimens, localized in the nucleus of tubular epithelial cells, glomerular mesangial cells, as well as infiltrated interstitial lymphocytes.

CONCLUSION

HBV infection closely related with IgAN and HBV infection might be involved in pathogenesis of IgAN.

摘要

目的

阐明乙型肝炎病毒（HBV）感染与IgA肾病（IgAN）之间的关系。

方法

采用免疫组织化学技术检测IgAN患者肾组织中的HBV抗原（HBAg），通过Southern印迹分析和原位杂交确定肾组织中HBV DNA的携带状态和定位。

结果

100例IgAN患者中有18例（18%）血清HBsAg检测阳性，100例患者中有31例（31%）肾组织检测到HBAg，其中31例患者中有20例（65%）肾小球检测到HBAg，31例患者中有10例（32%）同时检测到HBsAg和HBcAg。在肾小管上皮细胞（45%，14/31）和肾间质（6%，2/31）中也分别检测到HBcAg。Southern印迹分析证实6例患者中有5例肾组织中整合型HBV DNA呈阳性。原位杂交显示，所有8个标本的肾小管和肾小球中HBV DNA均为8/8和6/8阳性，定位于肾小管上皮细胞、肾小球系膜细胞以及浸润的间质淋巴细胞的细胞核中。