Zhang J, Wang N, Deng B
Chongqing Children's Hospital, Chongqing Medical University, Chongqing 400014, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2001 Aug;22(4):300-3.
To study VP7 type of rotavirus from children with diarrhea in Chongqing area from 1998 to 2000.
Double-strand RNA of rotavirus extracted from stool samples was used as the template for reverse transcription of gene VP7, which was followed by nested PCR for VP7 typing. Nucleotide sequence analysis was used for VP7 typing.
Among the 130 fecal specimens from pediatric patients with acute gastroenteritis during the epidemic season of 1998-1999, fifty specimens (38.46%) were identified as rotavirus-positive by RT-PCR. Of these, serotype G1 was found to be largely predominant and accounted for 88% (44/50), while serotype G3 accounted for 8% (4/50), coinfection of G1 and G3 accounted for 4% (2/50) respectively. While during the next epidemic season from 1999 to 2000, thirty-eight (33.93%) rotavirus positive specimens were detected from 112 stool samples by RT-PCR. Serotype G3 increased significantly-up to 78.95% (30/38) and became the most prevalent serotype in that season. It was found that only 13.16% (5/38) were serotype G1, 7.89% (3/38) were G1 and G3 mixture. The nucleotide sequence analysis results agreed with the RT-PCR typing assay.
Serotype G1 and G3 were the prevalent VP7 types in Chongqing, China from 1998 to 2000. A shift from G1 to G3 was observed in the last two successive epidemic seasons of rotavirus epidemics.
研究1998年至2000年重庆地区腹泻儿童的轮状病毒VP7型。
以从粪便样本中提取的轮状病毒双链RNA为模板进行基因VP7的逆转录，随后进行VP7分型的巢式PCR。采用核苷酸序列分析进行VP7分型。
在1998 - 1999年流行季节收集的130份小儿急性胃肠炎粪便标本中，经RT-PCR检测，50份（38.46%）为轮状病毒阳性。其中，G1型血清型占主导地位，占88%（44/50），G3型血清型占8%（4/50），G1和G3型混合感染分别占4%（2/50）。而在接下来的1999 - 2000年流行季节，从112份粪便样本中通过RT-PCR检测到38份（33.93%）轮状病毒阳性标本。G3型血清型显著增加，高达78.95%（30/38），成为该季节最流行的血清型。发现只有13.16%（5/38）为G1型血清型，7.89%（3/38）为G1和G3型混合感染。核苷酸序列分析结果与RT-PCR分型检测结果一致。
1998年至2000年，G1型和G3型是中国重庆地区流行的VP7型。在轮状病毒流行的最后两个连续流行季节中观察到了从G1型向G3型的转变。
