Berois Mabel, Libersou Sonia, Russi José, Arbiza Juan, Cohen Jean
UMR CNRS-INRA Virologie Moléculaire et Structurale, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
J Med Virol. 2003 Nov;71(3):456-62. doi: 10.1002/jmv.10511.
The gene encoding the protein VP7 that induces the major neutralizing response has been sequenced from 34 human rotaviruses isolated from children with acute diarrhea in Montevideo (Uruguay) over a 4-year period (1996-1999). These sequences were analyzed and compared to representative corresponding sequences available on databases. In most years, serotype G1 was present as the single serotype, except in 1999 when serotypes G1 and G4 were present simultaneously. Two G1 VP7 lineages were identified. Serotype G2 was present in 1997. The G4 isolates are grouped with Argentine strains and emerged during 1998 in a recently defined sublineage. Neither serotype G3 nor the emerging serotype G9 were isolated during the study. Antigenic domains of isolates and of representative reference strains of each serotype were compared. Sequences of strains isolated during the same year, showed a high degree of homology among strains belonging to the same serotype.
在4年期间（1996 - 1999年），从蒙得维的亚（乌拉圭）患有急性腹泻的儿童中分离出34株人轮状病毒，对编码诱导主要中和反应的蛋白质VP7的基因进行了测序。分析了这些序列，并与数据库中可用的代表性相应序列进行了比较。在大多数年份，G1血清型是唯一存在的血清型，1999年除外，当时G1和G4血清型同时存在。鉴定出两个G1 VP7谱系。G2血清型在1997年出现。G4分离株与阿根廷菌株归为一组，并于1998年出现在一个最近定义的亚谱系中。在研究期间未分离到G3血清型或新出现的G9血清型。比较了各血清型分离株和代表性参考菌株的抗原结构域。同年分离的菌株序列显示，属于同一血清型的菌株之间具有高度同源性。
