Robinson M W, Colhoun L M, Fairweather I, Brennan G P, Waite J H
Fascioliasis Research Group, The School of Biology and Biochemistry, The Queen's University of Belfast, Northern Ireland.
Parasitology. 2001 Nov;123(Pt 5):509-18. doi: 10.1017/s0031182001008630.
The development of the vitellaria of Fasciola hepatica within the liver of its rat host was studied by means of whole-mount stained preparations and transmission electron microscopy, together with light and electron immunocytochemistry using an antibody to vitelline protein B, an eggshell precursor protein synthesized by F. hepatica. No vitelline cells could be identified in flukes recovered from the liver parenchyma, by any of the methods used. In contrast, follicles were present in flukes at the earliest time of recovery from the bile duct, namely, 5 weeks 3 days post-infection. The vitellaria in these flukes formed a row of small follicles on either side of the body. Development of the follicles was rapid: by 6 weeks 3 days, the vitellaria resembled those in the adult fluke and eggs were present in the uterus. Immunolabelling was confined to the shell protein globules in the vitelline cells, confirming the packaging of the eggshell protein within the shell globule clusters.
通过整装染色标本、透射电子显微镜，以及使用针对卵黄蛋白B（一种由肝片吸虫合成的卵壳前体蛋白）的抗体进行光镜和电镜免疫细胞化学研究，对肝片吸虫在大鼠宿主体内肝脏中的卵黄腺发育进行了研究。使用任何一种方法，均未在从肝实质中回收的吸虫中鉴定出卵黄细胞。相比之下，在从胆管中最早回收吸虫的时间（即感染后5周3天），其体内就存在滤泡。这些吸虫中的卵黄腺在虫体两侧形成一排小滤泡。滤泡发育迅速：到6周3天时，卵黄腺类似于成虫吸虫中的卵黄腺，子宫内有虫卵。免疫标记仅限于卵黄细胞中的壳蛋白小球，证实了卵壳蛋白在壳小球簇中的包装。
