Vilcek S
University of Veterinary Medicine, Kosice, Slovak Republic.
Acta Virol. 2001 Apr;45(2):81-6.
Three pestiviruses in fetal calf serum (FCS), a pestivirus in porcine ST cell line, and two pestiviruses in two of five bovine cell lines were detected by RT-PCR method employing panpestivirus primers selected from the 5'-non-coding region (5'-NCR). The 288 bp products were sequenced in both directions. To identify these pestiviruses, their nucleotide sequences and those of reference pestiviruses were used for construction of a dendrogram. Three viruses present in FCS and a virus present in the bovine RP-15 cell line were identified as bovine viral diarrhea virus I (BVDV-1). Bovine viral diarrhea virus 2 (BVDV-2) was identified in a batch of the bovine MDBK cell line. A pestivirus contaminating the porcine ST cell line was identified as a Border disease virus (BDV).
使用从5'-非编码区（5'-NCR）中选择的泛瘟病毒引物，通过RT-PCR方法在胎牛血清（FCS）中检测到三种瘟病毒，在猪ST细胞系中检测到一种瘟病毒，在五个牛细胞系中的两个细胞系中检测到两种瘟病毒。对288 bp的产物进行双向测序。为了鉴定这些瘟病毒，将它们的核苷酸序列与参考瘟病毒的核苷酸序列用于构建系统发育树。FCS中存在的三种病毒和牛RP-15细胞系中存在的一种病毒被鉴定为牛病毒性腹泻病毒1型（BVDV-1）。在一批牛MDBK细胞系中鉴定出牛病毒性腹泻病毒2型（BVDV-2）。污染猪ST细胞系的一种瘟病毒被鉴定为边界病病毒（BDV）。
