Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy.
Res Vet Sci. 2012 Feb;92(1):147-50. doi: 10.1016/j.rvsc.2010.10.015. Epub 2010 Nov 19.
Recently, atypical bovine pestiviruses (BVDV-3) have been identified in batches of contaminated foetal calf serum (FCS) and in naturally infected cattle. During routine screening of FCS by conventional panpestivirus PCR assay, one batch showed traces of pestivirus nucleic acids, and the contaminating virus was typed as BVDV-3-like. Phylogenetic analysis based on three genome regions (5'UTR, N(pro) and E2) showed that this strain, named IZSPLV_To, clusters in a separate clade with CH_KaHo/cont, a cell culture contaminant detected in Switzerland. This study is the first report of the detection in Italy of a FCS batch contaminated with BVDV-3 and adds more evidence that atypical pestiviruses represent a serious cause for concern in cell culture laboratories, with potential repercussions on BVD control and vaccine biosafety. Our findings suggest that the BE/B2 primers may be able to detect BVDV-3 in a panpestivirus assay, but testing of a larger number of strains is required.
最近，在一批受污染的胎牛血清（FCS）和自然感染的牛中发现了非典型牛瘟病毒（BVDV-3）。在常规 panpestivirus PCR 检测中，对 FCS 进行常规筛查时，发现一批样品存在痕量瘟病毒核酸，污染的病毒被鉴定为 BVDV-3 样。基于三个基因组区域（5'UTR、N(pro) 和 E2）的系统进化分析表明，该株命名为 IZSPLV_To，与在瑞士检测到的细胞培养污染物 CH_KaHo/cont 聚集在一个单独的分支中。本研究首次在意大利报告了 FCS 批次受到 BVDV-3 污染，这进一步证明了非典型瘟病毒是细胞培养实验室中一个严重的问题，可能对 BVD 控制和疫苗生物安全产生影响。我们的研究结果表明，BE/B2 引物可能能够在 panpestivirus 检测中检测到 BVDV-3，但需要对更多的菌株进行测试。
