Postgraduate Program in Biotechnology, Federal University of São Carlos, São Carlos, SP, Brazil.
Lasers Med Sci. 2012 Sep;27(5):1059-65. doi: 10.1007/s10103-011-1045-4. Epub 2012 Jan 17.
Topical glucocorticoid (GC) therapy has been successfully used in the treatment of several common cutaneous diseases in clinical practice for a long time, and skin atrophy is one of the most typical cutaneous side effects of this therapy. The aim of this study was to evaluate the potential of noninvasive fluorescence spectroscopy (FS) technique in the detection and classification of GC-induced skin atrophy. A total of 20 male Wistar rats were used in the experimental protocol under controlled environmental conditions and with free access to food. One group received topical application of clobetasol propionate 0.05% for 14 days to induce cutaneous atrophy (atrophic group) and the other (control) group received only vehicle application following the same protocol and schedule. Histological analyses and FS measurements with laser excitation at both 532 nm and 408 nm were obtained on days 1 and 15. The FS results were classified as "normal" or "atrophic" according by histological analysis. Fluorescence spectra obtained with excitation at 408 nm allowed a clear distinction between the control and atrophic groups, and were more informative than the those obtained at 532 nm. Our results reveal that, if correctly applied, FS allows noninvasive evaluation of corticosteroid-induced skin atrophy, and thus represents an important step towards better monitoring of undesirable side effects of cutaneous therapy.
局部糖皮质激素（GC）治疗在临床上已成功用于治疗多种常见皮肤病，皮肤萎缩是该治疗最典型的皮肤副作用之一。本研究旨在评估非侵入性荧光光谱（FS）技术在检测和分类 GC 诱导的皮肤萎缩中的潜在应用。在受控环境条件下和自由进食的情况下，实验方案共使用了 20 只雄性 Wistar 大鼠。一组接受丙酸氯倍他索 0.05%的局部应用，持续 14 天，以诱导皮肤萎缩（萎缩组），另一组（对照组）在相同的方案和时间表下仅接受载体应用。在第 1 天和第 15 天进行组织学分析和激光激发 532nm 和 408nm 下的 FS 测量。根据组织学分析，FS 结果被分类为“正常”或“萎缩”。用 408nm 激发获得的荧光光谱能够清楚地区分对照组和萎缩组，并且比用 532nm 激发获得的光谱更具信息量。我们的结果表明，如果正确应用，FS 允许对皮质类固醇诱导的皮肤萎缩进行非侵入性评估，因此代表了朝着更好地监测皮肤治疗不良副作用迈出的重要一步。
