日本单一VP4血清型的G3B和G14马A组轮状病毒占主导地位。
Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan.
作者信息
Tsunemitsu H, Imagawa H, Togo M, Shouji T, Kawashima K, Horino R, Imai K, Nishimori T, Takagi M, Higuchi T
机构信息
Shichinohe Research Unit, National Institute of Animal Health, Aomori, Japan.
出版信息
Arch Virol. 2001 Oct;146(10):1949-62. doi: 10.1007/s007050170044.
A total of 65 equine group A rotaviruses (GAR) isolated from diarrheal foals at 48 farms in Hokkaido, Japan, between 1996 (29 isolates) and 1997 (36 isolates) were characterized for their VP7 and VP4 serotypes by PCR, nucleotide sequencing, and virus neutralization (VN) tests. By PCR VP7 typing, all isolates were classified as G3 or G 14, and the predominant serotype in each year was G3 (86%) in 1996 and G14 (53%) in 1997. VN tests with these 20 isolates randomly selected confirmed the specificity of PCR on the bases of complete agreement of the results in these methods (9 G3 and 11 G14), and revealed that all 9 G3 isolates were subtype G3B. There were five differing amino acid residues in three VP7 antigenic regions between subtypes G3A and G3B. Antiserum to a baculovirus recombinant that expressed P[12] VP4 neutralized all isolates and P[12] reference strains. These results suggest that genotype P[12] GAR belong to a single VP4 serotype, and that one VP4 and two VP7 serotypes (G3B and G14) of GAR were predominant in the equine population in Japan.
1996年（29株分离株）至1997年（36株分离株）期间，从日本北海道48个农场腹泻马驹中分离出65株马A组轮状病毒（GAR），通过聚合酶链反应（PCR）、核苷酸测序和病毒中和（VN）试验对其VP7和VP4血清型进行了鉴定。通过PCR VP7分型，所有分离株被归类为G3或G14，每年的主要血清型分别为1996年的G3（86%）和1997年的G14（53%）。对随机选择的这20株分离株进行的VN试验，基于这些方法结果的完全一致（9株G3和11株G14），证实了PCR的特异性，并显示所有9株G3分离株均为G3B亚型。G3A和G3B亚型之间的三个VP7抗原区域存在五个不同的氨基酸残基。表达P[12]VP4的杆状病毒重组体的抗血清中和了所有分离株和P[12]参考毒株。这些结果表明，基因型P[12]GAR属于单一VP4血清型，并且GAR的一种VP4和两种VP7血清型（G3B和G14）在日本马群中占主导地位。
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