Lowery C J, Moore J E, Millar B C, McCorry K A, Xu J, Rooney P J, Dooley J S
Northern Ireland Public Health Laboratory, Belfast, UK.
J Appl Microbiol. 2001 Nov;91(5):774-9. doi: 10.1046/j.1365-2672.2001.01440.x.
To investigate the incidence and genotype of Cryptosporidium parvum oocysts in drinking water sources in Northern Ireland for the period 1996-1999, and to compare conventional and molecular methods of detection.
Four hundred and seventy-four waters were investigated by conventional methods, namely immuno-fluorescent antibody detection (IFA; 380) and immuno-magnetic separation-IFA (IMS-IFA; 94), of which 14/474 (3%) were positive. Two hundred and fourteen samples (214/474) were also investigated by PCR techniques, targeting both the 18S rRNA and TRAP-C2 genes, of which 11/214 (5.1%) were positive. These 11 samples were classified as genotype II following sequence analysis of the TRAP-C2 amplicon.
This study demonstrated the low incidence of oocysts of C. parvum in water sources in Northern Ireland.
Such molecular-based techniques offer a number of advantages over conventional detection methodologies, namely greater sensitivity and specificity as well as the ability to provide accurate genotyping data rapidly, which may be valuable in directing operational management in potential outbreak situations.
调查1996 - 1999年北爱尔兰饮用水源中小隐孢子虫卵囊的发生率和基因型，并比较传统检测方法和分子检测方法。
采用传统方法对474份水样进行调查，即免疫荧光抗体检测（IFA；380份）和免疫磁珠分离 - IFA（IMS - IFA；94份），其中14/474（3%）呈阳性。还采用聚合酶链反应（PCR）技术对214份样本（214/474）进行检测，靶向18S核糖体RNA（rRNA）和TRAP - C2基因，其中11/214（5.1%）呈阳性。对TRAP - C2扩增子进行序列分析后，将这11份样本归类为II型基因型。
本研究表明北爱尔兰水源中小隐孢子虫卵囊的发生率较低。
此类基于分子的技术相对于传统检测方法具有诸多优势，即更高的灵敏度和特异性，以及能够快速提供准确的基因分型数据，这在潜在疫情情况下指导运营管理方面可能具有重要价值。
