Lowery C J, Millar B C, Moore J E, Xu J, Xiao L, Rooney P J, Crothers L, Dooley J S
Department of Bacteriology, Belfast City Hospital, School of Biological and Environmental Sciences, Faculty of Life and Health Sciences, University of Ulster, Coleraine, Northern Ireland.
Ir J Med Sci. 2001 Oct-Dec;170(4):246-50. doi: 10.1007/BF03167789.
Cryptosporidium parvum is the most common of the protozoal pathogens associated with gastrointestinal disease in Northern Ireland. Genotyping techniques are valuable in helping to elucidate sources and modes of transmission of this parasite. There have been no reports on the prevalence of genotypes in Northern Ireland, mainly due to a lack of discriminatory genotyping techniques, which recently have become available.
To investigate the genotype of C. parvum oocysts isolated from human faeces in sporadic cases of cryptosporidiosis in Northern Ireland.
Thirty-nine isolates of C. parvum, representing 79.6% of the total 1998 laboratory reports for the Eastern Health and Social Services Board, were investigated. Following DNA extraction from oocysts the thrombospondin-related adhesive protein 2 (TRAP-C2) locus was amplified by polymerase chain reaction (PCR) and subsequently sequenced.
The majority of isolates (87.2%) were classified as bovine genotype II with the remainder (12.8%) being the human genotype I.
There is a high prevalence of the bovine genotype II parasite in sporadic cases around the greater Belfast area. Epidemiologically, this suggests that the most frequent mode of transmission may be from animals to humans, but does not suggest a high proportion of human to human spread.
微小隐孢子虫是北爱尔兰与胃肠道疾病相关的最常见原生动物病原体。基因分型技术有助于阐明这种寄生虫的来源和传播方式。北爱尔兰此前没有关于基因型流行情况的报告，主要原因是缺乏具有鉴别力的基因分型技术，而这种技术最近已经出现。
调查从北爱尔兰散发性隐孢子虫病病例的人类粪便中分离出的微小隐孢子虫卵囊的基因型。
对39株微小隐孢子虫分离株进行了研究，这些分离株占东部卫生和社会服务委员会1998年实验室报告总数的79.6%。从卵囊中提取DNA后，通过聚合酶链反应（PCR）扩增血小板反应蛋白相关粘附蛋白2（TRAP-C2）基因座，随后进行测序。
大多数分离株（87.2%）被归类为牛基因型II，其余（12.8%）为人类基因型I。
在大贝尔法斯特地区周围的散发病例中，牛基因型II寄生虫的流行率很高。从流行病学角度来看，这表明最常见的传播方式可能是从动物传播给人类，但并不意味着人与人之间的传播比例很高。
