Hatzinger P B, McClay K, Vainberg S, Tugusheva M, Condee C W, Steffan R J
Envirogen, Inc., Lawrenceville, NJ 08648, USA.
Appl Environ Microbiol. 2001 Dec;67(12):5601-7. doi: 10.1128/AEM.67.12.5601-5607.2001.
Biodegradation of methyl tert-butyl ether (MTBE) by the hydrogen-oxidizing bacterium Hydrogenophaga flava ENV735 was evaluated. ENV735 grew slowly on MTBE or tert-butyl alcohol (TBA) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the addition of a small amount of yeast extract. The addition of H(2) did not enhance or diminish MTBE degradation by the strain, and MTBE was only poorly degraded or not degraded by type strains of Hydrogenophaga or hydrogen-oxidizing enrichment cultures, respectively. MTBE degradation activity was constitutively expressed in ENV735 and was not greatly affected by formaldehyde, carbon monoxide, allyl thiourea, or acetylene. MTBE degradation was inhibited by 1-amino benzotriazole and butadiene monoepoxide. TBA degradation was inducible by TBA and was inhibited by formaldehyde at concentrations of >0.24 mM and by acetylene but not by the other inhibitors tested. These results demonstrate that separate, independently regulated genes encode MTBE and TBA metabolism in ENV735.
对氧化氢细菌黄氢噬胞菌ENV735对甲基叔丁基醚（MTBE）的生物降解作用进行了评估。ENV735在MTBE或叔丁醇（TBA）作为唯一碳源和能源的情况下生长缓慢，但添加少量酵母提取物可显著促进其在这些底物上的生长。添加H₂对该菌株降解MTBE没有增强或减弱作用，并且嗜氢菌的模式菌株或氧化氢富集培养物分别对MTBE降解很差或不能降解。MTBE降解活性在ENV735中组成型表达，并且不受甲醛、一氧化碳、烯丙基硫脲或乙炔的显著影响。MTBE降解受到1-氨基苯并三唑和丁二烯单环氧化物的抑制。TBA降解可被TBA诱导，并且在浓度>0.24 mM时受到甲醛和乙炔的抑制，但不受其他测试抑制剂的抑制。这些结果表明，在ENV735中，编码MTBE和TBA代谢的基因是分开的、独立调控的。
