Hamamura N, Storfa R T, Semprini L, Arp D J
Department of Botany and Plant Pathology, Construction, and Environmental Engineering, Oregon State University, Corvallis, Oregon 97331-2902, USA.
Appl Environ Microbiol. 1999 Oct;65(10):4586-93. doi: 10.1128/AEM.65.10.4586-4593.1999.
Butane monooxygenases of butane-grown Pseudomonas butanovora, Mycobacterium vaccae JOB5, and an environmental isolate, CF8, were compared at the physiological level. The presence of butane monooxygenases in these bacteria was indicated by the following results. (i) O(2) was required for butane degradation. (ii) 1-Butanol was produced during butane degradation. (iii) Acetylene inhibited both butane oxidation and 1-butanol production. The responses to the known monooxygenase inactivator, ethylene, and inhibitor, allyl thiourea (ATU), discriminated butane degradation among the three bacteria. Ethylene irreversibly inactivated butane oxidation by P. butanovora but not by M. vaccae or CF8. In contrast, butane oxidation by only CF8 was strongly inhibited by ATU. In all three strains of butane-grown bacteria, specific polypeptides were labeled in the presence of [(14)C]acetylene. The [(14)C]acetylene labeling patterns were different among the three bacteria. Exposure of lactate-grown CF8 and P. butanovora and glucose-grown M. vaccae to butane induced butane oxidation activity as well as the specific acetylene-binding polypeptides. Ammonia was oxidized by all three bacteria. P. butanovora oxidized ammonia to hydroxylamine, while CF8 and M. vaccae produced nitrite. All three bacteria oxidized ethylene to ethylene oxide. Methane oxidation was not detected by any of the bacteria. The results indicate the presence of three distinct butane monooxygenases in butane-grown P. butanovora, M. vaccae, and CF8.
对以丁烷为生长底物的食烷假单胞菌、母牛分枝杆菌JOB5和一株环境分离菌CF8的丁烷单加氧酶进行了生理水平的比较。这些细菌中存在丁烷单加氧酶可由以下结果表明。（i）丁烷降解需要氧气。（ii）丁烷降解过程中产生1-丁醇。（iii）乙炔抑制丁烷氧化和1-丁醇生成。对已知的单加氧酶失活剂乙烯和抑制剂烯丙基硫脲（ATU）的反应区分了这三种细菌的丁烷降解情况。乙烯使食烷假单胞菌的丁烷氧化不可逆失活，但对母牛分枝杆菌或CF8无此作用。相反，仅CF8的丁烷氧化受到ATU的强烈抑制。在所有三株以丁烷为生长底物的细菌中，在存在[¹⁴C]乙炔的情况下标记了特定的多肽。这三种细菌的[¹⁴C]乙炔标记模式不同。将以乳酸为生长底物的CF8和食烷假单胞菌以及以葡萄糖为生长底物的母牛分枝杆菌暴露于丁烷中，可诱导丁烷氧化活性以及特定的乙炔结合多肽。所有三种细菌都能氧化氨。食烷假单胞菌将氨氧化为羟胺，而CF8和母牛分枝杆菌产生亚硝酸盐。所有三种细菌都将乙烯氧化为环氧乙烷。未检测到任何一种细菌有甲烷氧化现象。结果表明，在以丁烷为生长底物的食烷假单胞菌、母牛分枝杆菌和CF8中存在三种不同的丁烷单加氧酶。
