Hernandez-Perez G, Fayolle F, Vandecasteele J P
Institut Français du Pétrole, Département de Microbiologie, Rueil-Malmaison.
Appl Microbiol Biotechnol. 2001 Jan;55(1):117-21. doi: 10.1007/s002530000482.
Gordonia terrae strain IFP 2001 was selected from activated sludge for its capacity to grow on ethyl t-butyl ether (ETBE) as sole carbon and energy source. ETBE was stoichiometrically degraded to t-butyl alcohol (TBA) and the activity was inducible. A constitutive strain, G. terrae IFP 2007, derived from strain IFP 2001, was also selected. Methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) were not used as carbon and energy sources by the two strains, but cometabolic degradation of MTBE and TAME was demonstrated, to TBA and t-amyl alcohol (TAA) respectively, in the presence of a carbon source such as ethanol. No two-carbon compound was detected during growth on ETBE, but formate was produced during cometabolic degradation of MTBE or TAME. A monooxygenase was involved in the degradation of ethers, because no degradation of ETBE was observed under anaerobic conditions and the presence of a cytochrome P-450 was demonstrated in G. terrae IFP 2001 after induction by cultivation on ETBE.
戈登氏菌属的地戈登氏菌菌株IFP 2001是从活性污泥中筛选出来的，因为它能够以乙基叔丁基醚（ETBE）作为唯一的碳源和能源生长。ETBE按化学计量比降解为叔丁醇（TBA），且该活性是可诱导的。还筛选出了一株源自菌株IFP 2001的组成型菌株——地戈登氏菌IFP 2007。这两株菌都不能将甲基叔丁基醚（MTBE）和叔戊基甲基醚（TAME）用作碳源和能源，但在乙醇等碳源存在的情况下，证明了它们对MTBE和TAME的共代谢降解，分别降解为TBA和叔戊醇（TAA）。在以ETBE为碳源生长期间未检测到二碳化合物，但在MTBE或TAME的共代谢降解过程中产生了甲酸。一种单加氧酶参与了醚的降解，因为在厌氧条件下未观察到ETBE的降解，并且在以ETBE培养诱导后，在地戈登氏菌IFP 2001中证明了细胞色素P - 450的存在。
