Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates.
作者信息
Kapanidis A N, Ebright Y W, Ebright R H
机构信息
Howard Hughes Medical Institute, Waksman Institute and Department of Chemistry, Rutgers University Piscataway New Jersey 08854.
出版信息
J Am Chem Soc. 2001 Dec 5;123(48):12123-5. doi: 10.1021/ja017074a.