Wartmann T, Gellissen G, Kunze G
Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
Curr Genet. 2001 Oct;40(3):172-8. doi: 10.1007/s002940100254.
Oxygen influences the synthesis of mitochondrial proteins by alteration of the expression of mitochondrial genes and several nuclear genes. One of the genes localised in the nucleus is the EFG1 gene that encodes the mitochondrial elongation factor G (MEF-G). This unique gene (AEFG1) has been isolated from the non-conventional dimorphic yeast, Arxula adeninivorans LS3. The AEFG1 gene comprises a ORF of 2,274 bp, which corresponds to 757 amino acids. In the present study, the regulation of AEFG1 has been analysed for different morphological stages of A. adeninivorans and various culture conditions. It was demonstrated that the transfer of aerobically growing cultures to anaerobic conditions resulted in an accumulation of AEFG1 transcript, correlating with an increase in AMEF-G protein concentration. Since this regulation occurred in budding-cell culture growing at 30 degrees C and in both of the mycelial cultures grown at 45 degrees C and 30 degrees C, respectively, it was the oxygen level (but not the cultivation temperature or the morphological stage) which influenced the AEFG1 regulation.
氧气通过改变线粒体基因和一些核基因的表达来影响线粒体蛋白质的合成。位于细胞核中的一个基因是EFG1基因，它编码线粒体延伸因子G（MEF-G）。这个独特的基因（AEFG1）已从非常规双态酵母阿氏腺囊菌LS3中分离出来。AEFG1基因包含一个2274 bp的开放阅读框，对应757个氨基酸。在本研究中，已针对阿氏腺囊菌的不同形态阶段和各种培养条件分析了AEFG1的调控。结果表明，将需氧生长的培养物转移至厌氧条件会导致AEFG1转录本的积累，这与AMEF-G蛋白浓度的增加相关。由于这种调控发生在30℃下生长的芽殖细胞培养物以及分别在45℃和30℃下生长的两种菌丝体培养物中，所以是氧气水平（而非培养温度或形态阶段）影响了AEFG1的调控。
