Tømmeraas K, Strand S P, Tian W, Kenne L, Vårum K M
Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Carbohydr Res. 2001 Dec 7;336(4):291-6. doi: 10.1016/s0008-6215(01)00275-0.
Chitosans with chemical composition ranging from a fraction of N-acetylated units (F(A)) of 0.01 to 0.61 were used to prepare fluorescence labelled chitosans by reductive amination with 9-anthraldehyde. Fluorescent chitosans with a low theoretical degree of substitution (DS, 0.001-1%) were prepared, and the actual DS of the products were determined by UV and (1)H NMR spectroscopy. The fluorescence excitation and emission spectra of the chitosan with F(A) of 0.09 and DS 1% showed an excitation maximum at 254 nm and an emission maximum at 413 nm. The intrinsic viscosities ([eta]) of the fluorescent chitosans were compared to those of the original chitosans, showing that the derivatisation procedure lead only to a negligible decrease in [eta]. The conformation of these fluorescent chitosans with very low DS-values is not altered and they can conveniently be directly quantified by UV or fluorescence spectroscopy.
使用N - 乙酰化单元（F(A)）含量在0.01至0.61之间的壳聚糖，通过与9 - 蒽醛进行还原胺化反应来制备荧光标记的壳聚糖。制备了理论取代度（DS）较低（0.001 - 1%）的荧光壳聚糖，并通过紫外光谱和（1）H核磁共振光谱测定产物的实际DS。F(A)为0.09且DS为1%的壳聚糖的荧光激发光谱和发射光谱显示，其最大激发波长为254 nm，最大发射波长为413 nm。将荧光壳聚糖的特性粘度（[η]）与原始壳聚糖的特性粘度进行比较，结果表明衍生化过程只会导致[η]出现可忽略不计的下降。这些DS值非常低的荧光壳聚糖的构象未发生改变，并且可以通过紫外光谱或荧光光谱方便地直接进行定量分析。
