Oláh I, Nagy N, David C, Gumati M K, Magyar A
Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
Ital J Anat Embryol. 2001;106(2 Suppl 1):271-7.
A panel of monoclonal antibodies was produced to study the mesenchymal stromal elements of the bursa of Fabricius in guinea hen. The intracellular antigens recognized by GIIF3 and NIC2 monoclonal antibodies are of 50 and 30 kD, respectively. The cells identified by these antibodies emerge in the mesenchyme around day 12 of incubation, and immigrate into the surface epithelium of the bursal folds, which precedes the follicle formation. The GIIF3 cells move up to the luminal surface of the follicle and differentiate to follicle-associated epithelial cells. The NIC2 cells remain in the medulla, produce and secrete large amount NIC2 positive substance, when the bursal function starts up. The presence of double positive (GIIF3 and NIC2) cells in the medulla around hatching, seems to indicate, that the two cells might have a common origin. The NIC2 positive product of the bursal secretory dendritic cells contributes to the microenvironment, and possibly necessary for the B cell clonal expansion and establisment of the immune repertoire in the guinea hen.
制备了一组单克隆抗体，用于研究珍珠鸡法氏囊的间充质基质成分。GIIF3和NIC2单克隆抗体识别的细胞内抗原分别为50kD和30kD。这些抗体识别的细胞在孵化第12天左右出现在间充质中，并迁移到法氏囊皱襞的表面上皮，这发生在卵泡形成之前。GIIF3细胞向上移动到卵泡的腔面并分化为卵泡相关上皮细胞。当法氏囊功能启动时，NIC2细胞留在髓质中，产生并分泌大量NIC2阳性物质。孵化前后髓质中双阳性（GIIF3和NIC2）细胞的存在似乎表明，这两种细胞可能有共同的起源。法氏囊分泌树突状细胞的NIC2阳性产物有助于微环境的形成，可能是珍珠鸡B细胞克隆扩增和免疫库建立所必需的。
