Nagy Nándor, Magyar Attila, Tóth Miklós, Oláh Imre
Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
J Morphol. 2004 Mar;259(3):328-39. doi: 10.1002/jmor.10199.
The quail is the chimeric and parabiotic counterpart of the chicken, thus increasing the value of quail in the field of developmental biology. Quail bursa of Fabricius was studied by light microscopy, electron microscopy, and immunocytochemical methods. The basic cellular composition and structural framework are comparable with those of the chicken bursa. One of the major structural differences is the absence of the continuous cortico-medullary arch. In addition to the epithelial reticular cell the bursal secretory dendritic cell is the other medullary-specific bursal cell. The bursal secretory dendritic cell is a highly elongated cell which expresses vimentin intermediate filaments and produces secretory granules. The substance of the granules can be visualized by NIC2 monoclonal antibody, which was produced against guinea fowl bursal secretory dendritic cell. The released granular content appears on the lateral surface of the bursal secretory dendritic cell and is gradually solubilized. Thus, the NIC2-positive substance may occur in membrane-bound and solubilized forms in the isolated environment of the medulla. The bursal secretory dendritic cell establishes membrane contact areas with the B cells; therefore, they may influence B-cell maturation by cell contact and chemical (humoral) product. During embryogenesis bursal secretory dendritic cell precursors enter the epithelium and 1) induce epithelial bud formation, and 2) produce an NIC2-positive substance. Senescent bursal secretory dendritic cells can be phagocytic and migrate into the follicle-associated epithelium. This physiological turnover of the bursal secretory dendritic cell represents a novel pathway of macrophage formation from dendritic cells.
鹌鹑是鸡的嵌合体和联体生物对应物，因此提高了鹌鹑在发育生物学领域的价值。采用光学显微镜、电子显微镜和免疫细胞化学方法对鹌鹑法氏囊进行了研究。其基本细胞组成和结构框架与鸡的法氏囊相当。主要结构差异之一是没有连续的皮质-髓质弓。除上皮网状细胞外，囊分泌树突状细胞是另一种髓质特异性囊细胞。囊分泌树突状细胞是一种高度伸长的细胞，表达波形蛋白中间丝并产生分泌颗粒。颗粒物质可用针对珍珠鸡囊分泌树突状细胞产生的NIC2单克隆抗体进行可视化观察。释放的颗粒内容物出现在囊分泌树突状细胞的侧面并逐渐溶解。因此，NIC2阳性物质可能以膜结合和溶解形式存在于髓质的隔离环境中。囊分泌树突状细胞与B细胞建立膜接触区域；因此，它们可能通过细胞接触和化学（体液）产物影响B细胞成熟。在胚胎发生过程中，囊分泌树突状细胞前体进入上皮并1）诱导上皮芽形成，以及2）产生NIC2阳性物质。衰老的囊分泌树突状细胞可具有吞噬作用并迁移至滤泡相关上皮。囊分泌树突状细胞的这种生理更新代表了从树突状细胞形成巨噬细胞的新途径。
