Nagy Nándor, Oláh Imre
Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, 1094 Budapest, Tuzoltó str. 58., Hungary.
J Immunol Methods. 2009 Apr 15;343(2):97-102. doi: 10.1016/j.jim.2009.01.013. Epub 2009 Mar 3.
Bursa of Fabricius is a primary lymphoid organ in the birds and its microenvironment is responsible for the B cell maturation. The epithelial anlage develops through epithelial-mesenchymal interaction into which -as a third party - hemopoietic cells immigrate. Chemical bursectomy can be made by dipping or injecting eggs by testosterone solution. Both methods of treatments compromise the development of the whole embryo. Heparin beads of 70-150 microm in diameter were soaked in 5% testosterone solution and implanted into the tail bud of 4 day old chicken embryos before the bursal anlage began to develop at embryonic day 5. The locally applied testosterone inhibited the differentiation of the bursal secretory dendritic cell (BSDC) precursors, which were the inducer of the follicular epithelial bud formation. Testosterone treatment did not blocked the entering of B cells and other hemopoietic cells into the bursal mesenchyme, but in the absence of BSDC precursor cells the dendroepithelial microenvironment is not established, subsequently B cell precursors are lodged only in the bursal mesenchyme. This method is suitable for locally introducing other regulatory molecules, involved in bursal development.
法氏囊是鸟类的主要淋巴器官，其微环境负责B细胞的成熟。上皮原基通过上皮-间充质相互作用发育而成，造血细胞作为第三方迁移到其中。化学去囊法可通过用睾酮溶液浸泡或注射鸡蛋来实现。这两种治疗方法都会损害整个胚胎的发育。将直径为70-150微米的肝素珠浸泡在5%的睾酮溶液中，并在胚胎第5天法氏囊原基开始发育之前植入4日龄鸡胚的尾芽中。局部应用的睾酮抑制了法氏囊分泌性树突状细胞（BSDC）前体的分化，而BSDC前体是滤泡上皮芽形成的诱导物。睾酮处理并未阻止B细胞和其他造血细胞进入法氏囊间充质，但在没有BSDC前体细胞的情况下，树突状上皮微环境无法建立，随后B细胞前体仅滞留在法氏囊间充质中。这种方法适用于局部引入参与法氏囊发育的其他调节分子。
