Clemente M, Garma C, De Sola B G, Henriques-Gil N
Unidad de Genética, Universidad San Pablo CEU, Montepríncipe, Boadilla del Monte, Madrid, Spain.
Hereditas. 2001;134(2):135-40. doi: 10.1111/j.1601-5223.2001.00135.x.
Restriction enzyme analysis of the mitochondrial DNA of Eyprepocnemis plorans with 9 restriction enzymes revealed low variability--only EcoRI revealed any variability with 3 distinguishable digestion patterns, here named types I, II and III. The samples studied were collected from regions where different types of B chromosome exist. The demes from the central area show the B1 type (as a relict of a probably once continuous distribution) and also mtDNA type II. These show a parallel substitution towards the SW neighbouring demes by other Bs and mtDNA type I. However, the latter mtDNA type had a wider distribution and is predominant, or even the only one found in most other samples where other Bs exist. Considering the mtDNA as a marker unlinked with the Bs, some genetic differences should exist among areas defined by the B chromosomes. Our results support the hypothesis that in the central region the B chromosomes and the mtDNA are involved in two different events of substitution, but these do not necessarily occur in the same way.
用9种限制酶对绿腿腹露蝗线粒体DNA进行的限制酶分析显示出低变异性——只有EcoRI显示出任何变异性，有3种可区分的消化模式，这里命名为I型、II型和III型。所研究的样本是从存在不同类型B染色体的区域收集的。来自中心区域的群落显示出B1型（作为可能曾经连续分布的遗迹）以及线粒体DNA II型。这些显示出向西南相邻群落平行替代为其他B染色体和线粒体DNA I型。然而，后一种线粒体DNA类型分布更广且占主导地位，甚至在存在其他B染色体的大多数其他样本中是唯一发现的类型。将线粒体DNA视为与B染色体不连锁的标记，由B染色体定义的区域之间应该存在一些遗传差异。我们的结果支持这样的假设，即在中心区域，B染色体和线粒体DNA参与了两种不同的替代事件，但这些事件不一定以相同的方式发生。
