Aramaki J, Löffler C, Kawana S, Effendy I, Happle R, Löffler H
Department of Dermatology, Nippon Medical School, Japan.
Br J Dermatol. 2001 Nov;145(5):704-8. doi: 10.1046/j.1365-2133.2001.04442.x.
It is well known that the degree of skin reaction to an irritant depends on its concentration and exposure time.
To determine the interrelationship between the concentration of sodium lauryl sulphate (SLS) and exposure time in both weak (subclinical) and severe reactions.
Patch testing with SLS was performed at different concentrations (0.125%, 0.25%, 0.5%, 1.0% and 2.0%) and with different exposure times (3, 6, 12, 24 and 48 h). Evaluation was conducted by measurement of transepidermal water loss and by laser-Doppler flowmetry both 30 min and 24 h after patch removal.
We found more reliable and constant skin reactions 24 h after patch removal, and a higher correlation between SLS concentration and skin reaction.
We conclude that the concentration of SLS influences the test outcome to a larger degree than the exposure time. We present formulae by which the outcome of SLS patch testing at various SLS concentrations ranging from 0.125% to 2% and any exposure time between 3 and 24 h can be estimated.
众所周知，皮肤对刺激物的反应程度取决于其浓度和接触时间。
确定月桂醇硫酸酯钠（SLS）浓度与接触时间在轻度（亚临床）和重度反应中的相互关系。
采用不同浓度（0.125%、0.25%、0.5%、1.0%和2.0%）的SLS进行斑贴试验，并设置不同的接触时间（3、6、12、24和48小时）。在去除斑贴后30分钟和24小时，通过测量经表皮水分流失和激光多普勒血流仪进行评估。
我们发现去除斑贴24小时后皮肤反应更可靠且稳定，SLS浓度与皮肤反应之间的相关性更高。
我们得出结论，SLS浓度对测试结果的影响程度大于接触时间。我们给出了公式，通过这些公式可以估算在0.125%至2%的各种SLS浓度以及3至24小时的任何接触时间下SLS斑贴试验的结果。
