Löffler H, Pirker C, Aramaki J, Frosch P J, Happle R, Effendy I
Department of Dermatology, Philipp University Marburg, Deutschhausstr. 9, D-35033 Marburg, Germany.
Eur J Dermatol. 2001 Sep-Oct;11(5):416-9.
Irritant patch testing with sodium lauryl sulfate (SLS) will become more and more a routine test determining skin susceptibility in men. Recently, it has been shown that for practical reasons, irritant SLS patch testing can take place on the back simultaneously with a routine allergic patch test to other contact allergens. However, SLS patch testing has mostly been performed on the forearm in studying experimental skin irritation so far. The aim of this study was to determine whether there is a relationship in skin response to aqueous SLS (0.125%; 0.25%; 0.5% and 1.0%) between the forearm and the back assessed by visual scoring and measurement of transepidermal water loss (TEWL). We found a pronounced reaction of the forearm compared to the back. TEWL values as well as visual scores correlated well with SLS concentration. There was also a high correlation in visual scoring between the forearm and the back. Based on test sensitivity and specificity we suggest a 48 hrs patch test for routine screening with 0.5% SLS on the forearm evaluated by TEWL measurement or visual scoring 24 hrs after patch removal. A mild erythema (scored as < or =1) is considered to be normal. If for practical reasons, the SLS patch is placed on the back simultaneously with the allergic patch test, 0.5% SLS may be sufficient, too. TEWL measurement so far provides a reliable method and will certainly be necessary for experimental studies on irritant skin reactions, particularly when different SLS concentrations are used. After a 48 hrs patch test with SLS 0.5% TEWL measurement should be performed at 72 hrs. A value of < or =31.6 g/m(2)hr seems to follow the normal distribution.
用十二烷基硫酸钠（SLS）进行刺激性斑贴试验将越来越成为一种用于确定男性皮肤易感性的常规试验。最近有研究表明，出于实际原因，刺激性SLS斑贴试验可与针对其他接触性变应原的常规变应性斑贴试验同时在背部进行。然而，迄今为止，在研究实验性皮肤刺激时，SLS斑贴试验大多在前臂进行。本研究的目的是通过视觉评分和经表皮水分流失（TEWL）测量来确定在前臂和背部对不同浓度（0.125%、0.25%、0.5%和1.0%）的水性SLS的皮肤反应之间是否存在关联。我们发现前臂的反应比背部更明显。TEWL值以及视觉评分与SLS浓度密切相关。前臂和背部的视觉评分之间也有高度相关性。基于试验敏感性和特异性，我们建议进行48小时斑贴试验用于常规筛查，在前臂使用0.5%的SLS，在去除斑贴24小时后通过TEWL测量或视觉评分进行评估。轻度红斑（评分为≤1）被认为是正常的。如果出于实际原因，将SLS斑贴与变应性斑贴试验同时贴于背部，0.5%的SLS可能也足够。迄今为止，TEWL测量提供了一种可靠的方法，对于刺激性皮肤反应的实验研究肯定是必要的，特别是当使用不同浓度的SLS时。在用0.5%的SLS进行48小时斑贴试验后，应在72小时进行TEWL测量。≤31.6 g/m²·hr的值似乎符合正态分布。
