复制叉处的核小体定位。
Nucleosome positioning at the replication fork.
作者信息
Lucchini R, Wellinger R E, Sogo J M
机构信息
Institute of Cell Biology, ETH Hönggerberg, CH-8093 Zürich, Switzerland.
出版信息
EMBO J. 2001 Dec 17;20(24):7294-302. doi: 10.1093/emboj/20.24.7294.
Abstract
In order to determine the time required for nucleosomes assembled on the daughter strands of replication forks to assume favoured positions with respect to DNA sequence, psoralen cross-linked replication intermediates purified from preparative two-dimensional agarose gels were analysed by exonuclease digestion or primer extension. Analysis of sites of psoralen intercalation revealed that nucleosomes in the yeast Saccharomyces cerevisiae rDNA intergenic spacer are positioned shortly after passage of the replication machinery. Therefore, both the 'old' randomly segregated nucleosomes as well as the 'new' assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands, suggesting that the positioning of nucleosomes is an initial step in the chromatin maturation process.
摘要
为了确定在复制叉子链上组装的核小体相对于DNA序列占据有利位置所需的时间，通过核酸外切酶消化或引物延伸分析了从制备性二维琼脂糖凝胶中纯化的补骨脂素交联复制中间体。补骨脂素嵌入位点的分析表明，酿酒酵母rDNA基因间隔区中的核小体在复制机制通过后不久就定位了。因此，“旧的”随机分离的核小体以及“新的”组装的组蛋白八聚体都在新复制的DNA链上迅速（在数秒内）定位自身，这表明核小体的定位是染色质成熟过程中的初始步骤。
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