Mahmoud M R, El-Abhar H S, Saleh S
Department of Pharmacology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt.
J Ethnopharmacol. 2002 Jan;79(1):1-11. doi: 10.1016/s0378-8741(01)00310-5.
It has been reported that Nigella sativa oil possesses anticestode and antinematode actions. Besides, it produced a hepatoprotective effect in some models of liver toxicity. Therefore, our aim in this work was to study the effect of the Nigella oil (N.O) on Schistosomiasis mansoni infected mice. The oil was given in two dose levels (2.5 and 5 ml/kg, orally for two weeks) either alone or in combination with praziquantel (PZQ), the drug of choice for the treatment of schistosomiasis. Three aspects of drug action were investigated, the effect on Schistosomiasis mansoni infection, the effect on liver functions, and on redox state. The parasitological investigation included worm distribution, oogram pattern and ova count. Furthermore, liver granuloma diameters were measured. The biochemical parameters were the serum level of L-alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase (AP), albumin (Alb) and total protein. Moreover, to assess the antioxidant capability of the Nigella oil, four parameters were studied, viz., liver lipid peroxide (LPD) and reduced glutathione (GSH) contents and the activity of the defence enzyme superoxide dismutase (SOD) and lactate dehydrogenase (LDH). When the oil was given alone, it reduced the number of S. mansoni worms in the liver and decreased the total number of ova deposited in both the liver and the intestine. Furthermore, it increased the number of dead ova in the intestinal wall and reduced the granuloma diameters markedly. When N.O was administered in combination with PZQ, the most prominent effect was a further lowering in the dead ova number over that produced by PZQ alone. Concerning the biochemical parameters, infection of mice with S. mansoni produced a pronounced elevation in the serum activity of ALT, GGT, with a slight increase in AP level. However, it tended to reduce serum albumin level. These changes were accompanied with an alteration in the liver contents of LPD and GSH along with a significant decline in the activity of the cytosolic SOD and LDH. Administration of Nigella sativa oil succeeded partially to correct the previous changes in ALT, GGT, AP activity, as well as the Alb content in serum. However, it failed in the liver to restore either LPD and GSH content or LDH and SOD activities to normal level. These results suggest that Nigella sativa oil may play a role against the alterations caused by S. mansoni infection, an effect which may be induced partly by improving the immunological host system and to some extent with its antioxidant effect.
据报道，黑种草油具有抗绦虫和抗线虫作用。此外，在一些肝毒性模型中它还具有肝脏保护作用。因此，我们这项工作的目的是研究黑种草油（N.O）对曼氏血吸虫感染小鼠的影响。该油以两种剂量水平（2.5和5毫升/千克，口服两周）单独给药或与吡喹酮（PZQ）联合给药，吡喹酮是治疗血吸虫病的首选药物。研究了药物作用的三个方面，即对曼氏血吸虫感染的影响、对肝功能的影响以及对氧化还原状态的影响。寄生虫学调查包括蠕虫分布、虫卵图谱模式和虫卵计数。此外，还测量了肝脏肉芽肿直径。生化参数包括血清L-丙氨酸转氨酶（ALT）、γ-谷氨酰转移酶（GGT）、碱性磷酸酶（AP）、白蛋白（Alb）和总蛋白水平。此外，为了评估黑种草油的抗氧化能力，研究了四个参数，即肝脏脂质过氧化物（LPD）和还原型谷胱甘肽（GSH）含量以及防御酶超氧化物歧化酶（SOD）和乳酸脱氢酶（LDH）的活性。当单独给予该油时，它减少了肝脏中曼氏血吸虫的数量，并减少了肝脏和肠道中沉积的虫卵总数。此外，它增加了肠壁中死虫卵的数量，并显著减小了肉芽肿直径。当黑种草油与吡喹酮联合给药时，最显著的效果是死虫卵数量比单独使用吡喹酮时进一步降低。关于生化参数，曼氏血吸虫感染小鼠导致血清ALT、GGT活性显著升高，AP水平略有升高。然而，它倾向于降低血清白蛋白水平。这些变化伴随着肝脏中LPD和GSH含量的改变以及胞质SOD和LDH活性的显著下降。给予黑种草油部分成功地纠正了先前ALT、GGT、AP活性以及血清中Alb含量的变化。然而，它未能使肝脏中的LPD和GSH含量以及LDH和SOD活性恢复到正常水平。这些结果表明，黑种草油可能对曼氏血吸虫感染引起的改变起作用，这种作用可能部分是通过改善宿主免疫系统以及在一定程度上通过其抗氧化作用诱导的。
