Cabradilla C D, Toliver A P
Biochim Biophys Acta. 1975 Aug 21;402(2):188-98. doi: 10.1016/0005-2787(75)90038-6.
DNA - nuclear membrane complexes were isolated from HeLa cells and examined by either zone sedimentation analysis or isopycnic centrifugation in sucrose/CsCl gradients. The data suggest that the complexes formed during the first 10 min of the S-phase remain as stable structures throughout the cell cycle. Other DNA - nuclear membrane complexes are formed at later times during replication. These later complexes appear as multiple species and the association of DNA and the nuclear membrane seems to be of a transient nature. Together, these results suggest that both the replicative origins and the replication points of the DNA are associated with the nuclear membrane. Although the complexes formed at the start of the S-phase and at later times during the S-phase appear to differ, these differences may provide them with the needed properties to serve as spatial organizers for the temporal regulation of DNA replication.
从HeLa细胞中分离出DNA-核膜复合物，并通过区带沉降分析或在蔗糖/CsCl梯度中进行等密度离心来进行检测。数据表明，在S期的前10分钟形成的复合物在整个细胞周期中都保持为稳定结构。其他DNA-核膜复合物在复制后期形成。这些后期形成的复合物呈现出多种类型，并且DNA与核膜的结合似乎是短暂的。这些结果共同表明，DNA的复制起点和复制点都与核膜相关。尽管在S期开始时和S期后期形成的复合物似乎有所不同，但这些差异可能赋予它们所需的特性，使其能够作为DNA复制时间调控的空间组织者。
