Lagrue G, Xheneumont S, Branellec A, Hirbec G, Weil B
Biomedicine. 1975 Feb 10;23(1):37-40.
P.H.A. and Concanavalin A stimulated lymphocytes culture supernatants were comparatively studied in 23 volontary normals and in 46 patients with nephrotic syndrome. Intradermal injections to guinea-pig demonstrated a permeability increasing and pro-inflammatory factor. Vascular permeability (immediate reaction) is determinated by blue Evans technique. Delayed reaction is assesed by the cutaneous inflammatory process (erythema, induration); pathologically it is defined as polymorphonuclear and mononuclear infiltration. Differences are highly significant between nephrotic patients and normal subjects. This biological activity is not observed with control supernatants (medium plus normal human serum and P.H.A. or Conca A). Positive results were also found with M.E.M. eagle culture supernatants of nephrotic stimulated lymphocytes. To date lymphokines have been studied especially in animals. In human, they have been found only after concentration. Variations in lymphokine production may be present in some pathologic states.
对23名健康志愿者和46名肾病综合征患者的PHA和伴刀豆球蛋白A刺激的淋巴细胞培养上清液进行了比较研究。对豚鼠进行皮内注射显示出一种通透性增加和促炎因子。血管通透性（即时反应）通过伊文思蓝技术测定。迟发性反应通过皮肤炎症过程（红斑、硬结）进行评估；病理上定义为多形核和单核浸润。肾病患者与正常受试者之间的差异非常显著。对照上清液（培养基加正常人血清以及PHA或伴刀豆球蛋白A）未观察到这种生物活性。肾病刺激淋巴细胞的伊格尔MEM培养基培养上清液也得到了阳性结果。迄今为止，淋巴因子尤其在动物中得到了研究。在人类中，仅在浓缩后才发现它们。某些病理状态下可能存在淋巴因子产生的变化。
