Sobel A T, Branellec A I, Blanc C J, Lagrue G A
J Immunol. 1977 Oct;119(4):1230-4.
Stimulation of human lymphocytes with Con A resulted in the liberation of a soluble lymphokine that increased the permeability of guinea pig skin capillaries. This factor, termed vascular permeability factor (VPF), was characterized by physicochemical methods. Upon gel filtration chromatography on Sephadex G-100, it eluted in a narrow peak with an apparent molecular weight of 12,000 daltons. The sedimentation rate of VPF, estimated by sucrose density gradient ultracentrifugation, was 1.8 S. Polyacrylamide gel electrophoresis showed the biologic activity to migrate in the beta region; the factor displayed a pI of 6.4 upon isoelectric focusing. These characteristics allow VPF to be distinguished from other human lymphokines.
用刀豆蛋白A刺激人淋巴细胞会导致一种可溶性淋巴因子的释放，该因子可增加豚鼠皮肤毛细血管的通透性。这种被称为血管通透性因子（VPF）的因子通过物理化学方法进行了表征。在Sephadex G - 100上进行凝胶过滤色谱分析时，它以一个窄峰洗脱，表观分子量为12,000道尔顿。通过蔗糖密度梯度超速离心法估算，VPF的沉降速率为1.8 S。聚丙烯酰胺凝胶电泳显示其生物活性在β区域迁移；等电聚焦时该因子的等电点为6.4。这些特性使得VPF能够与其他人类淋巴因子区分开来。
