Bobadilla-del-Valle M, Ponce-de-Leon A, Arenas-Huertero C, Vargas-Alarcon G, Kato-Maeda M, Small P M, Couary P, Ruiz-Palacios G M, Sifuentes-Osornio J
Laboratory of Clinical Microbiology, Instituto Nacional de Ciencas Médicas y Nutritión Salvador Zubiran, Mexico City, Mexico.
Emerg Infect Dis. 2001 Nov-Dec;7(6):1010-3. doi: 10.3201/eid0706.010615.
The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using the DNA sequencing of the core region of rpoB for comparison. Of the RIFr, 24 had a PCR-SSCP pattern identical to that of H37Rv; the other 11 had four different patterns. The 11 RIFs had PCR-SSCP patterns identical to that of H37Rv. The sensitivity of the assay was 31.4%; its specificity was 100%. We observed a strong correlation between the degree of resistance and the type of mutation.
运用聚合酶链反应-单链构象多态性分析（PCR-SSCP）技术研究耐利福平（RIFr）结核分枝杆菌的rpoB基因突变，结果存在矛盾。为确定该方法的敏感性，我们分析了35株耐利福平菌株和11株利福平敏感（RIFs）菌株，并对rpoB核心区域进行DNA测序以作比较。在耐利福平菌株中，24株的PCR-SSCP图谱与H37Rv相同；另外11株有四种不同图谱。11株利福平敏感菌株的PCR-SSCP图谱与H37Rv相同。该检测方法的敏感性为31.4%；特异性为100%。我们观察到耐药程度与突变类型之间存在密切关联。
