Jakobsen Anne-Marie, Ahlman Håkan, Wängberg Bo, Kölby Lars, Bengtsson Malin, Nilsson Ola
Lundberg Laboratory for Cancer Research, Department of Pathology, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden.
J Pathol. 2002 Jan;196(1):44-50. doi: 10.1002/path.1002.
Identification of neuroendocrine differentiation in tumours has important implications for prognosis and therapy. The aim of the present study was to evaluate monoclonal antibodies against synaptic vesicle protein 2 (SV2) as histopathological markers for neuroendocrine differentiation in tumours of the gastrointestinal tract and pancreas. Paraffin blocks from 211 gastrointestinal tumours were examined by immunocytochemistry, using a monoclonal antibody against SV2. Virtually all endocrine tumours of the gastrointestinal tract (11/11 gastric, 53/53 ileal, 16/21 appendiceal, and 22/22 rectal) and pancreas (24/24) were positively labelled. SV2 labelling was also demonstrated in gastrointestinal pacemaker cell tumours (8/8), while adenocarcinomas of the gastrointestinal tract and pancreas were negative, with the exception of occasional adenocarcinomas demonstrating weak SV2 labelling (stomach 1/22, rectum 1/29, and pancreas 0/21). Western blotting of tumour biopsies confirmed expression of SV2 in endocrine tumours of the gastrointestinal tract and pancreas. No relationship was observed between SV2 expression in tumours and hormone production or malignant potential. In conclusion, SV2 is expressed in neuroendocrine tumours of the gastrointestinal tract and pancreas, but not in non-endocrine tumours. The SV2 monoclonal antibody can therefore be used as a general marker for neuroendocrine differentiation in gastrointestinal and pancreatic tumours.
肿瘤中神经内分泌分化的鉴定对预后和治疗具有重要意义。本研究的目的是评估针对突触小泡蛋白2（SV2）的单克隆抗体作为胃肠道和胰腺肿瘤中神经内分泌分化的组织病理学标志物。使用抗SV2单克隆抗体，通过免疫细胞化学检查了211例胃肠道肿瘤的石蜡块。几乎所有胃肠道（11/11例胃、53/53例回肠、16/21例阑尾和22/22例直肠）和胰腺（24/24例）的内分泌肿瘤均呈阳性标记。胃肠道起搏细胞肿瘤（8/8例）也显示SV2标记，而胃肠道和胰腺的腺癌为阴性，但偶尔有腺癌显示弱SV2标记（胃1/22例、直肠1/29例和胰腺0/21例）除外。肿瘤活检的蛋白质免疫印迹证实了SV2在胃肠道和胰腺内分泌肿瘤中的表达。未观察到肿瘤中SV2表达与激素产生或恶性潜能之间的关系。总之，SV2在胃肠道和胰腺的神经内分泌肿瘤中表达，但在非内分泌肿瘤中不表达。因此，SV2单克隆抗体可作为胃肠道和胰腺肿瘤中神经内分泌分化的通用标志物。
