Parija S C, Shivaprakash M R
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, 605 006, Pondicherry, India.
Diagn Microbiol Infect Dis. 2001 Nov;41(3):127-30. doi: 10.1016/s0732-8893(01)00300-5.
Potassium hydroxide (KOH) is widely used in the wet mount preparation of various clinical specimens for demonstration of fungi and fungal elements. In the present study we have evaluated the use of KOH in the wet mount preparation of fresh stool specimens for the demonstration of intestinal parasites. The KOH wet mount of stool was compared with saline, iodine and lacto-phenol cotton blue (LPCB) wet mount preparations of stool. A total of 855 fresh stool specimens were examined in the study of which 419 specimens were found to be positive for either trophozoites, cysts, ova or larvae of different parasites by all these methods. A total of 309 stool specimens were positive by KOH preparation of stool, while 289, 245 and 208 stool specimens were positive by LPCB, iodine and saline preparations respectively. The KOH was found to be effective in lysing the faecal debris, faecal mass and other artifacts without affecting many of the parasitic ova, cysts and larvae in the stool smears. The parasitic ova, cysts and larvae were impacted by the use of 10% KOH and could be easily visualized, detected and identified against a clear background in the stool smear I hr after preparation of KOH wet mount. From these findings we recommend the routine use of KOH wet mount of stool for demonstration of intestinal parasites in a parasitology laboratory.
氢氧化钾（KOH）广泛用于各种临床标本的湿片制备，以显示真菌和真菌成分。在本研究中，我们评估了KOH在新鲜粪便标本湿片制备中用于显示肠道寄生虫的情况。将粪便的KOH湿片与粪便的生理盐水、碘和石炭酸复红（LPCB）湿片制备进行了比较。在该研究中总共检查了855份新鲜粪便标本，通过所有这些方法发现其中419份标本对不同寄生虫的滋养体、包囊、虫卵或幼虫呈阳性。粪便的KOH制备有309份粪便标本呈阳性，而LPCB、碘和生理盐水制备分别有289、245和208份粪便标本呈阳性。发现KOH可有效溶解粪便残渣、粪块和其他假象，而不影响粪便涂片中的许多寄生虫卵、包囊和幼虫。使用10%KOH可使寄生虫卵、包囊和幼虫清晰可见，在制备KOH湿片1小时后，可在粪便涂片中清晰的背景下轻松观察、检测和识别。根据这些发现，我们建议在寄生虫学实验室常规使用粪便的KOH湿片来显示肠道寄生虫。
