Mank T G, Zaat J O, Blotkamp J, Polderman A M
General Practitioners Laboratory, Haarlem, Netherlands.
Eur J Clin Microbiol Infect Dis. 1995 Dec;14(12):1076-81. doi: 10.1007/BF01590942.
The use of sodium acetate acetic acid formalin (SAF)-preserved stool specimens was compared with that of nonpreserved specimens for the recovery of intestinal protozoa. A total of 247 patients, 170 with diarrhea of more than one week's duration and 77 refugees, were asked to collect a stool specimen. Each specimen was placed into two vials, one empty, the other containing SAF fixative. Laboratory investigations included microscopic examination of the concentrated sediment and direct wet smears from both types of stool specimens and the microscopic examination of a permanent stained smear from the unsedimented, SAF-preserved stool specimens. Examination of SAF-preserved stool specimens revealed intestinal protozoa in 149 of the 247 patients. With the conventional procedure using unpreserved stool specimens, intestinal protozoa were found in 89 of the 247 patients. The results show that the examination of SAF-preserved stool specimens, consisting of the microscopic examination of both the concentrated sediment and the permanent stained smear from the unsedimented material, increases the chance of recovering intestinal protozoa as compared to the conventional procedure.
将乙酸钠-乙酸-福尔马林（SAF）固定保存的粪便标本与未保存的标本用于肠道原生动物检测的效果进行了比较。共有247例患者参与，其中170例腹泻持续时间超过一周，77例为难民，要求他们采集粪便标本。每个标本分别放入两个小瓶中，一个为空瓶，另一个装有SAF固定剂。实验室检测包括对两种粪便标本的浓缩沉淀物和直接湿涂片进行显微镜检查，以及对未沉淀的SAF固定保存粪便标本的永久染色涂片进行显微镜检查。检查SAF固定保存的粪便标本发现，247例患者中有149例存在肠道原生动物。采用未保存粪便标本的传统方法检测时，247例患者中有89例发现肠道原生动物。结果表明，与传统方法相比，对SAF固定保存的粪便标本进行检测，即对浓缩沉淀物和未沉淀材料的永久染色涂片进行显微镜检查，可增加发现肠道原生动物的几率。
