Lenne-Samuel Nathalie, Wagner Jérôme, Etienne Hélène, Fuchs Robert P P
Institut de Recherche contre les Cancers de l'Appareil Digestif, UPR conventionnée de l'Université Louis Pasteur de Strasbourg, Hôpitaux Universitaires BP424, 67091 Strasbourg, France.
EMBO Rep. 2002 Jan;3(1):45-9. doi: 10.1093/embo-reports/kvf007. Epub 2001 Dec 19.
The dinB-encoded DNA polymerase IV (Pol IV) belongs to the recently identified Y-family of DNA polymerases. Like other members of this family, Pol IV is involved in translesion synthesis and mutagenesis. Here, we show that the C-terminal five amino acids of Pol IV are essential in targeting it to the beta-clamp, the processivity factor of the replicative DNA polymerase (Pol III) of Escherichia coli. In vivo, the disruption of this interaction obliterates the function of Pol IV in both spontaneous and induced mutagenesis. These results point to the pivotal role of the processivity clamp during DNA polymerase trafficking in the vicinity of damaged-template DNA.
由dinB基因编码的DNA聚合酶IV(Pol IV)属于最近鉴定出的Y家族DNA聚合酶。与该家族的其他成员一样,Pol IV参与跨损伤合成和诱变。在此,我们表明Pol IV的C末端五个氨基酸对于将其靶向β-钳至关重要,β-钳是大肠杆菌复制性DNA聚合酶(Pol III)的持续性因子。在体内,这种相互作用的破坏消除了Pol IV在自发诱变和诱导诱变中的功能。这些结果表明持续性钳在受损模板DNA附近的DNA聚合酶运输过程中起关键作用。