Kruse R A, Rubakhin S S, Romanova E V, Bohn P W, Sweedler J V
Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
J Mass Spectrom. 2001 Dec;36(12):1317-22. doi: 10.1002/jms.237.
Desorption/ionization on porous silicon (DIOS) is a form of laser desorption mass spectrometry that allows for the direct mass analysis of a variety of analytes without the addition of organic matrix. Protocols are described for the direct analysis of exocrine tissue and single neurons using DIOS-MS. The atrial gland of Aplysia californica was blotted on to porous silicon and analyzed with DIOS-MS in the range m/z 1000-4000. The ability to culture invertebrate neurons directly on porous silicon is also presented. Isolated bag cells regenerated neuronal processes in culture on porous silicon. DIOS-MS allowed the direct detection of the peptides contained in individual cultured neurons indicating that with appropriate protocols, DIOS can be used with biological samples with considerable thickness.
多孔硅解吸/电离（DIOS）是激光解吸质谱的一种形式，可在不添加有机基质的情况下对多种分析物进行直接质量分析。本文描述了使用DIOS-MS直接分析外分泌组织和单个神经元的实验方案。将加州海兔的心房腺印迹在多孔硅上，并用DIOS-MS在m/z 1000 - 4000范围内进行分析。还展示了在多孔硅上直接培养无脊椎动物神经元的能力。分离的袋状细胞在多孔硅上培养时可再生神经突起。DIOS-MS能够直接检测单个培养神经元中所含的肽，这表明通过适当的实验方案，DIOS可用于厚度可观的生物样品。
