单细胞代谢物和肽的分析。
Profiling metabolites and peptides in single cells.
机构信息
Department of Chemistry and the Beckman Institute, University of Illinois at Urbana-Champaign, Urbana-Champaign, Illinois, USA.
出版信息
Nat Methods. 2011 Apr;8(4 Suppl):S20-9. doi: 10.1038/nmeth.1549. Epub 2011 Mar 30.
Abstract
The intracellular levels and spatial localizations of metabolites and peptides reflect the state of a cell and its relationship to its surrounding environment. Moreover, the amounts and dynamics of metabolites and peptides are indicative of normal or pathological cellular conditions. Here we highlight established and evolving strategies for characterizing the metabolome and peptidome of single cells. Focused studies of the chemical composition of individual cells and functionally defined groups of cells promise to provide a greater understanding of cell fate, function and homeostatic balance. Single-cell bioanalytical microanalysis has also become increasingly valuable for examining cellular heterogeneity, particularly in the fields of neuroscience, stem cell biology and developmental biology.
摘要
细胞内代谢物和肽的水平和空间定位反映了细胞的状态及其与周围环境的关系。此外，代谢物和肽的数量和动态变化是正常或病理细胞状态的指示。在这里，我们重点介绍了用于描述单细胞代谢组和肽组的既定和不断发展的策略。对单个细胞和功能定义的细胞群的化学成分的重点研究有望提供对细胞命运、功能和动态平衡的更深入了解。单细胞生物分析微分析在检查细胞异质性方面也变得越来越有价值，特别是在神经科学、干细胞生物学和发育生物学领域。
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