Park J C, Park B J, Suh H, Park B Y, Rah D K
Department of Plastic and Reconstructive Surgery and Institute for Human Tissue Restoration, Yonsei University College of Medicine, C.P.O. Box 8044, Seoul 120-752, Korea.
Yonsei Med J. 2001 Dec;42(6):587-94. doi: 10.3349/ymj.2001.42.6.587.
One of the differences between fetal and adult skin healing is the ability of fetal wounds heal without contraction and scar formation. Extracellular matrix (ECM) provides a substratum for cells adhesion, migration, and proliferation and can directly influence the form and function of cells. As motility is essential for many important biological events, including wound healing, inflammatory response, embryonic development, and tumor metastasis, this study was designed to compare the motilities cultured dermal fetal and neonatal fibroblasts in the extracellular matrix. The motility of cultured fetal and neonatal fibroblasts was compared using a video-microscopy system that was developed in combination with a self-designed CO2 mini-incubator. To determine migration speed, cells were viewed with a 4X phase-contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. We found that cultured fetal fibroblasts move faster than neonatal fibroblast on type I collagen (fetal fibroblast, 15.1 micrometer/hr; neonatal fibroblast, 13.7 micrometer/hr), and in fibronectin (fetal fibroblast, 13.2 micrometer/hr; neonatal fibroblast, 13.0 micrometer/hr) and hyaluronic acid (fetal fibroblast, 11 micrometer/hr; neonatal fibroblast, 9.8 micrometer/hr).
胎儿皮肤愈合与成人皮肤愈合的差异之一在于胎儿伤口能够在不发生收缩和瘢痕形成的情况下愈合。细胞外基质（ECM）为细胞黏附、迁移和增殖提供了一个基质，并且可以直接影响细胞的形态和功能。由于运动性对于许多重要的生物学事件至关重要，包括伤口愈合、炎症反应、胚胎发育和肿瘤转移，本研究旨在比较培养的胎儿和新生儿真皮成纤维细胞在细胞外基质中的运动性。使用结合自行设计的二氧化碳微型培养箱开发的视频显微镜系统比较培养的胎儿和新生儿成纤维细胞的运动性。为了确定迁移速度，用4倍相差物镜观察细胞并进行视频记录。使用彩色电荷耦合器件（CCD）相机捕获图像，并以8位全彩色模式保存。我们发现，培养的胎儿成纤维细胞在I型胶原上（胎儿成纤维细胞，15.1微米/小时；新生儿成纤维细胞，13.7微米/小时）、纤连蛋白（胎儿成纤维细胞，13.2微米/小时；新生儿成纤维细胞，13.0微米/小时）和透明质酸（胎儿成纤维细胞，11微米/小时；新生儿成纤维细胞，9.8微米/小时）上的移动速度比新生儿成纤维细胞快。
