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[十足目甲壳动物精子中一种核外碱性蛋白的鉴定及细胞化学研究]

[Identification and cytochemical study of an extranuclear basic protein in the spermatozoa of decapod crustaceans].

作者信息

Chevaillier P

出版信息

J Cell Biol. 1967 Mar;32(3):547-56. doi: 10.1083/jcb.32.3.547.

Abstract

Extranuclear basic proteins have been detected in the capsule of the spermatozoa of three species of decapod crustaceans ( L., Macrura; L., Anomura; Penn., Brachyura). Their properties have been studied by cytochemical methods. Their position inside the capsule of the spermatozoon has been specified with the aid of the electron microscope. Present in a constant fashion in the three species cited, their relative importance is very variable. In contrast to the refringent cone of the spermatozoon of , which contains an acid protein, ascaradine, the capsule of the spermatozoon of the three decapod crustaceans studied contains basic proteins which we propose to designate by the general term "decapodine".

摘要

在三种十足目甲壳类动物(龙虾属,长尾亚目;寄居蟹属,异尾亚目;梭子蟹属,短尾亚目)精子的包膜中检测到了核外碱性蛋白。已通过细胞化学方法研究了它们的特性。借助电子显微镜确定了它们在精子包膜内的位置。在所提及的三种物种中以恒定方式存在,但其相对重要性差异很大。与蛔虫精子含酸性蛋白(ascaradine)的折光锥不同,所研究的三种十足目甲壳类动物精子的包膜含有碱性蛋白,我们建议用通用术语“十足目碱”来命名。

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HISTONE STAINING WITH AMMONIACAL SILVER.用氨性银进行组蛋白染色。
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