Coller J K, Fritz P, Zanger U M, Siegle I, Eichelbaum M, Kroemer H K, Mürdter T E
Dr Margarete Fischer-Bosch-Institut für Klinische Pharmakologie, Stuttgart, Germany.
Histochem J. 2001 Jun;33(6):329-36. doi: 10.1023/a:1012414806166.
Microsomal epoxide hydrolase is a biotransformation enzyme which is involved in the hydrolysis of various epoxides and epoxide intermediates. In the present study, its distribution was investigated in both normal human tissues and human tumours of different histogenetic origin using immunohistochemical techniques. In normal tissue, epithelial cells were more often and more intensely immunostained than mesenchymal cells. The main epithelial cell types expressing microsomal epoxide hydrolase were hepatocytes, acinus cells of the pancreas, and cells of salivary and adrenal glands. Immunostained cells of mesenchymal origin included monocytes, fibrocytes, fibroblasts, vessel endothelium, muscle cells, and cells of the reproductive system. Three patterns of expression were observed in tumour tissues: (1) moderate or strong in hepatocellular carcinomas, tumours of the adrenal gland, and theca-fibromas of the ovary; (2) inhomogeneous staining pattern of variable intensity in breast cancer, lung cancer, colorectal carcinomas, carcinoid tumours, and some tumours of mesenchymal origin; and (3) no expression in malignant melanomas, malignant lymphomas, and renal carcinomas. These data indicate that microsomal epoxide hydrolase expression is not restricted to tissue of any particular histogenetic origin. Nonetheless, immunohistochemical identification of microsomal epoxide hydrolase may be helpful in some well-defined histological settings, for example, confirmation of hepatocellular carcinoma.
微粒体环氧化物水解酶是一种生物转化酶，参与各种环氧化物和环氧化物中间体的水解反应。在本研究中，运用免疫组织化学技术对其在正常人体组织和不同组织发生来源的人类肿瘤中的分布情况进行了研究。在正常组织中，上皮细胞的免疫染色比间充质细胞更为常见且更为强烈。表达微粒体环氧化物水解酶的主要上皮细胞类型为肝细胞、胰腺腺泡细胞以及唾液腺和肾上腺的细胞。间充质来源的免疫染色细胞包括单核细胞、纤维细胞、成纤维细胞、血管内皮细胞、肌肉细胞以及生殖系统的细胞。在肿瘤组织中观察到三种表达模式：（1）在肝细胞癌、肾上腺肿瘤和卵巢卵泡膜纤维瘤中呈中度或强阳性；（2）在乳腺癌、肺癌、结直肠癌、类癌肿瘤以及一些间充质来源的肿瘤中呈强度不一的不均匀染色模式；（3）在恶性黑色素瘤、恶性淋巴瘤和肾癌中无表达。这些数据表明微粒体环氧化物水解酶的表达并不局限于任何特定组织发生来源的组织。尽管如此，微粒体环氧化物水解酶的免疫组织化学鉴定在某些明确的组织学情况下可能会有所帮助，例如肝细胞癌的确诊。
