Kurumatani M, Yagi K, Murata T, Tezuka M, Mander L N, Nishiyama M, Yama H
Biotechnology Research Center, The University of Tokyo, Japan.
Biosci Biotechnol Biochem. 2001 Oct;65(10):2311-4. doi: 10.1271/bbb.65.2311.
Antheridiogens in culture media of 6-week-old prothallia of two species of Schizaeaceous ferns, Lygodium microphyllum and Lygodium reticulatum, were analyzed by gas chromatography-mass spectrometry. In both species, the gibberellin A73 methyl ester (GA73-Me) was identified as the most abundant antheridiogen, and the methyl esters of GA9 and of several monohydroxy-GA73 derivatives were also detected. Since both species produced antheridiogens at a high level, they were classified into high-antheridiogen-producing ferns. The response to GA73-Me of gametophytes of both species is also discussed.
利用气相色谱-质谱联用技术对两种莎草蕨科蕨类植物小叶海金沙和网脉海金沙6周龄原叶体培养基中的成雄素进行了分析。在这两个物种中，赤霉素A73甲酯（GA73-Me）被鉴定为含量最丰富的成雄素，同时还检测到了GA9和几种单羟基GA73衍生物的甲酯。由于这两个物种都能大量产生成雄素，因此它们被归类为高产成雄素的蕨类植物。文中还讨论了这两个物种配子体对GA73-Me的反应。
