Nociti F H, Nogueira-Filho G R, Tramontina V A, Machado M A, Barros S P, Sallum E A, Sallum A W
Dept. of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, UNICAMP, Sao Paulo, Brazil.
J Periodontal Res. 2001 Dec;36(6):361-6. doi: 10.1034/j.1600-0765.2001.360603.x.
The present study investigated the effect of nicotine administration on periodontal breakdown resulting from ligature-induced periodontitis in rats. Twenty adult male Wistar rats were used. After anesthesia, a mandibular first molar was randomly assigned to receive a cotton ligature in the sulcular area while the contralateral tooth was left unligated. The animals were randomly assigned to one of the following treatments. of daily intraperitoneal injections: A - saline solution, B -0.37 mg of nicotine kg, C -0.57 mg of nicotine kg and D -0.73 mg of nicotine/kg. Thirty days later, the animals were sacrificed and the specimens routinely processed for serial decalcified sections. Statistical analysis (ANOVA) revealed greater bone loss (p<0.05) in the ligated teeth of animals which received nicotine (groups B/C D) than in the ligated teeth of animals which received saline solution (group A). In addition, a dose-dependent response was observed among the nicotine groups. A negative effect of nicotine was observed in the unligated teeth of the experimental groups (p<0.05). Therefore, daily administration of nicotine enhanced, in a dose-dependent manner, the effects of local factors in producing periodontal breakdown. Furthermore, the nicotine seemed to have a direct deleterious effect on the periodontal tissues.
本研究调查了给予尼古丁对结扎诱导的大鼠牙周炎所致牙周组织破坏的影响。使用了20只成年雄性Wistar大鼠。麻醉后，随机选择一侧下颌第一磨牙在龈沟区域放置棉线结扎，对侧牙齿不结扎。将动物随机分为以下治疗组之一，进行每日腹腔注射：A组 - 生理盐水，B组 - 0.37mg尼古丁/千克，C组 - 0.57mg尼古丁/千克，D组 - 0.73mg尼古丁/千克。30天后，处死动物，标本常规处理以制作系列脱钙切片。统计分析（方差分析）显示，接受尼古丁的动物（B/C/D组）结扎牙齿的骨丢失量比接受生理盐水的动物（A组）结扎牙齿的骨丢失量更大（p<0.05）。此外，在尼古丁组中观察到剂量依赖性反应。在实验组未结扎的牙齿中也观察到尼古丁的负面影响（p<0.05）。因此，每日给予尼古丁以剂量依赖性方式增强了局部因素导致牙周组织破坏的作用。此外，尼古丁似乎对牙周组织有直接的有害影响。
