Pereira Vasconcelos Daniel F, Dias da Silva Marco A, Rocha Marques Marcelo, Gibilini Cristina, Cardoso Guimarães Vasconcelos Any C, Pereira Barros Silvana
Division of Histology and Embryology, School of Biomedicine, Federal University of Piauí, Parnaíba-PI, Brazi.
Division of Histology and Embryology, Federal University of Campina Grande, Patos-PB, Brazil.
J Clin Exp Dent. 2013 Apr 1;5(2):e95-9. doi: 10.4317/jced.51047.
The aim of this study was to evaluate the effects of alcohol and nicotine, when used alone or simultaneously, on the alveolar bone loss area resulting from ligature-induced periodontitis in rats.
Forty adult male rats received a cotton ligature in the first lower molar sulcular area, and the animals were randomly assigned to different treatments (n = 10, each group) including daily peritoneal injections of saline solution (group A), submitted to self-administration of alcohol 25% (group B), nicotine solution in concentration 0.19 μl/ml (group C), and nicotine solution in concentration 0.19 μl/ml plus self-administration of alcohol 25% (group D). Five weeks later, the animals were sacrificed, and the samples were routinely processed for semi-serial decalcified sections.
Ligated teeth showed more alveolar bone loss than unligated ones (p < 0.05). Unligated teeth showed no significant differences between each other (p > 0.05). Analyses between the ligated teeth showed that the group C (nicotine) or group B (alcohol 25%) each had increasing alveolar bone loss in the furcation area, and the simultaneous combination alcohol and nicotine (group D) intensified these effects (p < 0.05).
The results suggest that the simultaneous combination of alcohol and nicotine have a synergistic effect in the progression of periodontitis, evidenced by increased furcation region bone destruction in periodontal disease in rats. Key words:Alveolar bone loss, periodontitis, nicotine, alcohol, rats.
本研究旨在评估单独或同时使用酒精和尼古丁对结扎诱导的大鼠牙周炎所致牙槽骨吸收面积的影响。
40只成年雄性大鼠在第一下颌磨牙龈沟区域放置棉线结扎，将动物随机分为不同处理组（每组n = 10），包括每日腹腔注射生理盐水（A组）、自行给予25%酒精（B组）、浓度为0.19 μl/ml的尼古丁溶液（C组）以及浓度为0.19 μl/ml的尼古丁溶液加自行给予25%酒精（D组）。5周后，处死动物，样本常规处理以制作半连续脱钙切片。
结扎牙的牙槽骨吸收比未结扎牙更多（p < 0.05）。未结扎牙之间无显著差异（p > 0.05）。对结扎牙的分析表明，C组（尼古丁）或B组（25%酒精）在根分叉区域的牙槽骨吸收均增加，酒精和尼古丁同时使用（D组）会强化这些影响（p < 0.05）。
结果表明，酒精和尼古丁同时使用在牙周炎进展中具有协同作用，大鼠牙周疾病根分叉区域骨破坏增加证明了这一点。关键词：牙槽骨吸收、牙周炎、尼古丁、酒精、大鼠
