Nogueira-Filho Getúlio R, Fróes Neto Edgard B, Casati Marcio Zaffalon, Reis Silvia R A, Tunes Roberto S, Tunes Urbino R, Sallum Enilson Antonio, Nociti Francisco H, Sallum Antonio W
Department of Periodontics, School of Dentistry, Bahia Science Foundation (FDC), Bahia, Brazil.
J Periodontol. 2004 Mar;75(3):348-52. doi: 10.1902/jop.2004.75.3.348.
The aim of the present study was to verify nicotine effects on alveolar bone changes induced by occlusal trauma during a periodontitis experimental model in rats.
Thirty adult male rats were used. The animals were randomly assigned to one of three groups receiving daily intraperitoneal injections: A, nicotine solution (0.44 mg/ml) and occlusal overload; B, saline solution and occlusal overload; or C, saline solution. Rats from groups A and B underwent bilateral amputation of the second and third molar cusps to simulate an occlusal overload. The first molars were then randomly assigned to receive a cotton ligature in the sulcular area, while the contralateral tooth was left unligated. The animals were sacrificed 30 days later. The resected mandibles were processed, and histomorphometric measurements were performed in the alveolar bone adjacent to the furcation area of the first molars.
Nicotine enhanced the bone loss induced by occlusal trauma (P<0.001) on the ligated teeth of group A (12.27 +/- 4.4 mm2), when compared to groups B (8.43 +/- 3.51 mm2) and C (4.43 +/- 2.17 mm2). Alveolar bone loss (P<0.01) was also observed in the contralateral teeth of groups A (nicotine + trauma) and B (saline + trauma), when compared to group C (saline only).
Within the limits of the study, it is concluded that nicotine may influence the alveolar bone changes induced by occlusal trauma by enhancing bone loss.
本研究的目的是在大鼠牙周炎实验模型中，验证尼古丁对咬合创伤诱导的牙槽骨变化的影响。
使用30只成年雄性大鼠。将动物随机分为三组，每组接受每日腹腔注射：A组，尼古丁溶液（0.44毫克/毫升）和咬合过载；B组，生理盐水溶液和咬合过载；或C组，生理盐水溶液。A组和B组的大鼠双侧切除第二和第三磨牙尖以模拟咬合过载。然后将第一磨牙随机分配在龈沟区域接受棉线结扎，而对侧牙齿不结扎。30天后处死动物。对切除的下颌骨进行处理，并在第一磨牙分叉区域相邻的牙槽骨中进行组织形态计量学测量。
与B组（8.43±3.51平方毫米）和C组（4.43±2.17平方毫米）相比，尼古丁增强了A组结扎牙齿上由咬合创伤诱导的骨丢失（P<0.001）（12.27±4.4平方毫米）。与C组（仅生理盐水）相比，A组（尼古丁+创伤）和B组（生理盐水+创伤）的对侧牙齿也观察到牙槽骨丢失（P<0.01）。
在本研究的范围内，得出结论：尼古丁可能通过增加骨丢失来影响由咬合创伤诱导的牙槽骨变化。
