Frölich K, Haerer G, Bacciarini L, Janovsky M, Rudolph M, Giacometti M
Institute for Zoo and Wildlife Research Berlin, Alfred-Kowalke-Strasse 17, P.O. Box 601103, 10252 Berlin, Germany.
J Wildl Dis. 2001 Oct;37(4):803-7. doi: 10.7589/0090-3558-37.4.803.
From 1997 to 2000, complete necropsy and histopathologic investigations were performed on 157 free-ranging European brown hares (Lepus europaeus) found dead throughout Switzerland. Organ samples of all these individuals (157 livers and 107 spleens available) were tested for European brown hare syndrome virus (EBHSV)-antigen by enzyme-linked immunosorbent assay (ELISA) test kit. Furthermore, 60 additional blood samples were tested for antibodies against EBHSV by ELISA. In addition, liver samples of 87 free-ranging mountain hares (Lepus timidus) hunted in 1996 were tested for EBHSV-antigen. In two European brown hares from southern Switzerland lesions suggestive of changes induced by EBHSV were present, and high titers of EBHSV-antigen were detected in both liver and spleen samples of these animals. Based on negative staining electron microscopy investigations of liver and spleen homogenates, we observed calicivirus in one antigen-positive hare. Low EBHSV-antigen titers were found in three additional European brown hares from central and western Switzerland, but EBHS-lesions were absent. Antibodies against EBHSV were not detected in any of the sera of European brown hares, and EBHSV-antigen was not found in the samples of mountain hares. This is the first report of EBHS in European brown hares from Switzerland.
1997年至2000年期间，对在瑞士各地发现死亡的157只野生欧洲棕兔（欧洲野兔）进行了完整的尸检和组织病理学调查。使用酶联免疫吸附测定（ELISA）试剂盒对所有这些个体的器官样本（可获得157个肝脏和107个脾脏）进行了欧洲棕兔综合征病毒（EBHSV）抗原检测。此外，还通过ELISA对另外60份血液样本进行了EBHSV抗体检测。另外，对1996年捕获的87只野生山地兔（白靴兔）的肝脏样本进行了EBHSV抗原检测。在瑞士南部的两只欧洲棕兔中，发现了提示由EBHSV引起变化的病变，并且在这些动物的肝脏和脾脏样本中均检测到高滴度的EBHSV抗原。基于对肝脏和脾脏匀浆的负染色电子显微镜检查，我们在一只抗原阳性的野兔中观察到了杯状病毒。在来自瑞士中部和西部的另外三只欧洲棕兔中发现了低EBHSV抗原滴度，但未发现EBHS病变。在欧洲棕兔的任何血清中均未检测到EBHSV抗体，并且在山地兔的样本中未发现EBHSV抗原。这是瑞士欧洲棕兔中EBHS的首次报告。
